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G.R. Barile, S.I. Pachydaki, S.R. Tari, S.E. Lee, L.L. Rong, L.G. Buciarelli, T. Wendt, C. Sakal, D.M. Stern, A.M. Schmidt; Accelerated Vascular Changes of Nonproliferative Diabetic Retinopathy in a Murine Model of Diabetes . Invest. Ophthalmol. Vis. Sci. 2003;44(13):3294.
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Purpose: To determine whether the induction of hyperlipidemia in the insulin-resistant hyperglycemic db/db mouse enhances the development of histopathologic features of nonproliferative diabetic retinopathy. Methods: ApoE null (ApoE-/-) mice bred into the db/db background (hyperlipidemic hyperglycemic HLHG; ApoE -/- db/db) were compared with normolipidemic normoglycemic (NLNG; ApoE +/+ m/db) age-matched littermate controls. Elastase digests of the retinas of 8 normolipidemic normoglycemic (NLNG; ApoE +/+ m/db) mice were compared with the digests of 10 hyperlipidemic normoglycemic mice (HLGL; ApoE-/- db/m), 5 normolipidemic hyperglycemic mice ( NLHG; ApoE+/+ db/db) and 10 hyperlipidemic hyperglycemic mice (HLHG; ApoE-/- db/db). The specimens were analysed for the presence of pericyte ghosts, acellular capillaries and microaneurysm formation. Statistical analyses were performed using ANOVA and post-hoc tests. Results: Both acellular capillaries and pericyte ghosts were increased in HLHG (ApoE -/- db/db) mice compared to the different contorl groups as shown in the table below. Conclusion: This murine model of combined insulin-resistant diabetes and hyperlipidemia demonstrates histological findings of nonproliferative diabetic retinopathy at 6 and 8 months of age. These data suggest that induction of hyperlipidemia in diabetic mice exacerbates vascular changes of nonproliferative diabetic retinopathy. View OriginalDownload SlideView OriginalDownload Slide
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