May 2003
Volume 44, Issue 13
ARVO Annual Meeting Abstract  |   May 2003
Expression of Cyclooxygenase-2 (COX-2) in Experimental Glaucoma in Rats
Author Affiliations & Notes
  • J.M. Kwong
    Jules Stein Eye Institute, Univ California Los Angeles, Los Angeles, CA, United States
  • J. Caprioli
    Jules Stein Eye Institute, Univ California Los Angeles, Los Angeles, CA, United States
  • T.T. Lam
    Doheny Eye Institute, Univ Southern California, Los Angeles, CA, United States
  • Footnotes
    Commercial Relationships  J.M. Kwong, None; J. Caprioli, None; T.T. Lam, None.
  • Footnotes
    Support  RPB (Dr Caprioli) and The Glaucoma Foundation (Dr Lam)
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 3320. doi:
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      J.M. Kwong, J. Caprioli, T.T. Lam; Expression of Cyclooxygenase-2 (COX-2) in Experimental Glaucoma in Rats . Invest. Ophthalmol. Vis. Sci. 2003;44(13):3320.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract: : Purpose: To examine the expression of COX-2 in the retina and the optic nerve head in a rat glaucoma model. Methods: Adult Sprague Dawley albino rats, 45-50 day-old, were given unilaterally intravitreal injection of India ink and trabecular laser photocoagulation was delivered at 7 days after injection. Intraocular pressure (IOP) was monitored. Eyes were enucleated at 0.5, 1, 3, 5, 7, and 14 days after photocoagulation. For a control group, laser photocoagulation was delivered to the mid-iris stroma after ink injection. The eyeballs were enucleated, bisected and processed for immunohistochemistry with antibody against COX-2 and TdT-mediated biotin-dUTP nick end labeling (TUNEL). A minimum of 6 eyes was examined for each group. Results: Significantly elevated IOP was noted 3 (69%), 5 (48%), and 7 (63%) days after photocoagulation when compared with the control contralateral eyes (P < 0.05; n = 61). There was a maximal number of TUNEL positive cells in the inner retinas at 5 days after photocoagulation (P < 0.01). Parallel to the increased number of TUNEL positive cells, there were scattered COX-2 immunopositive cells in the inner plexiform layer (IPL) and mildly increased COX-2 immunoreactivity in IPL at 5 and 7 days. There were no noticeable changes in other retinal layers and in the control retinas. In the optic nerve head, there were COX-2 immunoreactivity in control eyes and a generalized loss of immunoreactivity 14 days after photocoagulation. Conclusions: COX-2 may play an important role in the pathophysiology of experimental glaucoma.

Keywords: apoptosis/cell death • ganglion cells • retinal degenerations: cell biology 

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