May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
The Effect of BMP-4 on the Expression of BMP and TGFß Growth Factors and Their Receptors in Cells Isolated From the Human Optic Nerve Head
Author Affiliations & Notes
  • S.U. Das
    Anatomy & Pathology/Cell Bio&Gen, Univ of N Texas Hlth Sci Ctr, Fort Worth, TX, United States
  • W. Lambert
    Anatomy & Pathology/Cell Bio&Gen, Univ of N Texas Hlth Sci Ctr, Fort Worth, TX, United States
  • R. Agarwal
    Anatomy & Pathology/Cell Bio&Gen, Univ of N Texas Hlth Sci Ctr, Fort Worth, TX, United States
  • T. Tovar
    Anatomy & Pathology/Cell Bio&Gen, Univ of N Texas Hlth Sci Ctr, Fort Worth, TX, United States
  • X. Liu
    Anatomy & Pathology/Cell Bio&Gen, Univ of N Texas Hlth Sci Ctr, Fort Worth, TX, United States
  • A.F. Clark
    Glaucoma, Alcon Research Ltd., Fort Worth, TX, United States
  • R.J. Wordinger
    Glaucoma, Alcon Research Ltd., Fort Worth, TX, United States
  • Footnotes
    Commercial Relationships  S.U. Das, None; W. Lambert, None; R. Agarwal, None; T. Tovar, None; X. Liu, None; A.F. Clark, Alcon Research,Ltd. E; R.J. Wordinger, Alcon Research Ltd. F.
  • Footnotes
    Support  EY 12783
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 3331. doi:
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      S.U. Das, W. Lambert, R. Agarwal, T. Tovar, X. Liu, A.F. Clark, R.J. Wordinger; The Effect of BMP-4 on the Expression of BMP and TGFß Growth Factors and Their Receptors in Cells Isolated From the Human Optic Nerve Head . Invest. Ophthalmol. Vis. Sci. 2003;44(13):3331.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: Bone morphogenetic proteins (BMPs) were originally identified as osteoinductive cytokines but recently have been shown to be involved in development, morphogenesis, cell proliferation, and apoptosis of a wide variety of tissues and cells. BMPs are members of the TGFß superfamily and act through two distinct transmembrane serine/threonine kinase receptors (BMP-RI and BMP-RII). We have previously reported that BMPs and BMP receptors are expressed by lamina cribrosa cells (LC) and optic nerve head (ONH) astrocytes [(Wordinger et al, Mol. Vis. (2002) 8:241-250)]. Members of the BMP family function in a gene dosage manner during development and participate in ocular development. Heterozygous deficiency of BMP-4 results in anterior segment dysgenesis and elevated IOP. In glaucoma the ONH is altered and BMP-4 may play an important role by interacting with the other members of the TGFß family. The purpose is to determine the effect of BMP-4 treatment on the expression of BMPs, BMPRs, TGFß-2 and TGFß receptors in cells isolated from the human ONH. Methods: Recombinant BMP- 4 (20 ng/ml) was used to treat LC cells, and ONH astrocytes in serum free media for 72 hrs and compared with non-treated control. Total RNA was isolated and subjected to RT-PCR to examine the expression of mRNA for BMPs, BMP receptors, TGFß-2 and TGFß receptors in cultured human LC cells and ONH astrocytes. Western immunoblotting was used to study the expression of BMPs and TGFß-2 proteins. Results: Using semi-quantitative RT- PCR, BMP-4 treatment down-regulated mRNA for BMP-2, BMP-4, BMP-5, BMP-7, BMP-RIα, BMP-RIß, BMP-RII, TGFß-2 and TGFßR-II in the LC Cells. BMP-4 down regulated mRNA for BMP-4 and BMP-5 in ONH astrocytes. Conclusions: BMP-4 treatment causes down regulation of the BMPs, TGFß-2, and their receptors in cells isolated from the human ONH and may be involved in maintaining the normal microenvironment of the ONH. CR: P, F (RJW); E (AFC) Support: NIH Grant EY 12783 and Alcon Research Ltd., Fort Worth, TX.

Keywords: growth factors/growth factor receptors • lamina cribrosa • gene/expression 
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