Purchase this article with an account.
S.D. Grozdanic, Y.H. Kwon, D.M. Betts, R.A. Allbaugh, R.H. Kardon, D.S. Sakaguchi, I.M. Sonea; Laser-induced Mouse Model of Chronic Ocular Hypertension – A New Model for Glaucoma Studies . Invest. Ophthalmol. Vis. Sci. 2003;44(13):3334.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Purpose: To develop an inducible mouse model of glaucoma Methods: We induced obstruction of aqueous humor outflow in adult C57Bl6/J mice by combining injection of indocyanine green dye (ICG) into the anterior chamber, followed by diode laser treatment of the trabecular meshwork and episcleral veins enriched with the dye. Mice received 30-50 laser applications. To evaluate intraocular pressure (IOP), tonometry was performed with a modified Goldmann tonometer. The function of the retina was evaluated with electroretinography (ERG). Results: IOP was significantly elevated in 22 of 25 operated mice after laser-induced surgery compared to control (non-operated) eyes (preop=15.2+0.6mmHg; 10d postop=33.6+1.5 (p<0.001); 30d=27.4+1.2(p<0.001)). However, 60 days after surgery IOP in operated eyes decreased to 19.5+0.9mmHg and was not significantly different compared to control eyes (control=17.3+0.7mmHg, p=0.053). ERG amplitudes were decreased in operated eyes with predominant affect on inner retina function (b-wave amplitudes expressed as a ratio (operated/control): preop=107.6+4.6%, 28d postop=61+4% (p<0.001); 56d postop=62+5.6% (p<0.001)). Oscillatory potentials were the most dramatically affected: preop=108.6+6.7%; 28d=57.5+5% (p<0.01); 56d=57+8.5% (p<0.001). Amplitudes of the a-waves had relatively smaller but significant deficits: preop=105.8+6.9%, 28d=72.2+5.4% (p<0.01); 56d=79.8+11 (p<0.01). Flicker ERG analysis revealed mild but non-significant decrease of the function in operated eyes: prep=99+9%; 28d=78.5+13.3% (p>0.1); 56d=88.3+13.3% (p>0.1). Histological analysis of the operated eyes revealed development of anterior synechia, loss of retinal ganglion cells and thinning of all retinal layers with predominantly affects on retinal ganglion cell and inner plexiform layers. Electron microscopy of optic nerve cross sections revealed swelling and degeneration of the large diameter axons and evidence of gliosis. Conclusions: Diode laser treatment of ICG saturated episcleral veins causes a chronic elevation of the IOP and significant ERG deficits. Electroretinographic and histological analysis revealed primarily damage of the optic nerve and inner retina structures.
This PDF is available to Subscribers Only