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E. Ahmed, J. Ma, I. Rigas, N. Hafezi-Moghadam, E. Iliaki, E.S. Gragoudas, J.W. Miller, A.P. Adamis; Non-Invasive Tonometry in the Mouse . Invest. Ophthalmol. Vis. Sci. 2003;44(13):3336.
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Purpose: To develop and validate a non-invasive tonometer for the mouse eye Method: The principle of applanation tonometry was employed using a silicone MEMS-based (micro-electric-mechanical systems) fiber optic pressure sensor (Fiso Technologies). The pressure sensor applanated a fixed area of mouse cornea. The probe consisted of a silicone diaphragm bonded to a photolithographically etched Pyrex glass substrate enclosing a vacuum space that was attached to an optical fiber. The perpendicular distance of the vacuum space between the silicone diaphragm and the glass substrate varied inversely with the pressure. The two semi-reflective surfaces of the silicone and the glass acted as a Fabry-Perot interferometer, allowing the distance to be ascertained by analyzing their reflectance properties from a multiple frequency light source. The spatially spread light signal was recorded by photodetectors (sensitive to light intensity) positioned along the Fizeau interferometer. The tonometer was calibrated in the mouse eye by cannulating the mouse eye and manometrically measuring the IOP between the pressure range 10-80 mmHg. Results: The tonometrically measured IOP in the mouse correlated well with the manometric IOP (R2 = 0.92. The mean IOP measured in C57/B mice was 15.96+/-2.79 mmHg (mean + SD, n=10 ). Conclusions: A non-invasive tonometer has been developed for the mouse. This system permits serial non-invasive IOP measurement and can be used to evaluate mouse models of glaucoma.
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