Aqueous Humor Dynamics in Mouse

Abstract

Abstract: : Purpose: To determine the parameters of aqueous humor dynamics in mouse. Methods: NIH Swiss mice aged 8-16 weeks were used for the measurement of intraocular pressure (IOP), episcleral venous pressure (Pev), aqueous volume (Va), aqueous flow rate (Fa), and outflow facility (C) with various microneedle-based methods. Pev was measured by intentionally lowering intracameral pressure until the refluxed blood was observed in Schlemn’s canal. Va was measured as the aqueous fluid volume aspirated by a microneedle until there was iris-cornea contact. Fa was measured by determining the rate of dilution of intracamerally injected 10kda rhodamine-dextran. The value of C was calculated from flow rates measured at two different pressures. Conventional and uveoscleral outflow were estimated by the Goldmann equation. Results: Mouse aqueous humor dynamics  

Conclusions:Measurements of mouse aqueous humor dynamics are similar to those in human eyes. This supports the view that mouse is a useful model system for investigation of aqueous outflow.

Keywords: aqueous • intraocular pressure