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J.I. Clark, J.F. King, T.M. Seeberger, R.R. Ansari; Non-Invasive DLS Detects the Earliest Stages of Cataract and Inhibition of Lens Opacification by Pantethine In Vivo . Invest. Ophthalmol. Vis. Sci. 2003;44(13):3482.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To evaluate the sensitivity of fiber optic based dynamic light scattering (DLS) instrumentation in the early stages of opacification in vivo. Opacification in the selenite model and its inhibition by pantethine is well characterized temporally by slit lamp ophthalmoscopy. The reproducibility of the model makes the selenite cataract ideal for evaluating the earliest detectable stages of cataract formation. Methods: Rat pups (Charles River Laboratory) aged 12-14 days were injected subcutaneously with sodium selenite alone (Sigma, 3.28 mg/kg), pantethine (Sigma, 820 mg/kg) thirty minutes prior to sodium selenite, or saline. Animals were anesthetized with isoflurane gas and pupils were dilated with a 1:1 mixture of 10% Phenylephrine Hydrochloride and 1% Tropicamide. Body temperature was maintained by a heating pad and the cornea was kept hydrated with saline. In these experiments, visible light (670 nm wavelength) from a low power 80 micro-watt laser diode was focused into a small scattering volume on the optical axis of the lens of the living animal. Scattered light was collected by a fiber optic probe at 154 degrees angle from this scattering volume. DLS measurements were taken at 12, 16, 24, 36, 42, and 60 hours post injection. DLS scans were plotted graphically as intensity of light scattering as a function of spatial position along the optical axis of the lens. Slit lamp examinations were performed immediately following each DLS measurement. All animals were re-examined by slit lamp 8-15 days post-injection to determine whether a strong opacity had developed. Examinations were recorded by digital video and still photographs were captured with Adobe Premiere and processed with Adobe Photoshop. Results: Saline control and pantethine injected animals had similar DLS plots at all time points. None of these animals developed opacities when examined 1-2 weeks later. The intensity fluctuations with spatial position in lens increased by 36 hours after selenite injection. All selenite injected animals developed mature intense opacities by 1-2 weeks post injection. Conclusions: Animals that had a selenite injection alone showed significantly increased light scattering intensity in the lens by 36 hours. The fiber optic based DLS is very sensitive in evaluating the earliest stages of lens opacification prior to observation using slit lamp photography.
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