The Relationship for the Progression of Senile Cataracts and Blood-Aqueous Barrier Break

Abstract

Abstract: : Purpose: To evaluate the relationship for the progression of cataracts and blood -aqueous barrier, albumin levels in aqueous humor from senile cataract patients were microanalized and blood-aqueous barrier level was obtained by comparing with in their serum. Methods: Microanalysis of albumin was performed by capillary high performance liquid chromatography utilizing reverse phase micro column, Acetonitrile linear gradient (10-55%) and UV detector (280nm). Aqueous humor was obtained at cataract operation after informed consent. The ratio of aqueous humor and serum albumin for each patient was estimated as blood-aqueous barrier break. Results: Albumin levels were obtained 63.3 - 646.0µ g/ml (mean;243.7± 123.3µ g/ml) and the ratios of aqueous humor albumin levels to serum levels were 1/650-1/60 in 66 patients. An adequate correlation was obtained in the relationship of blood-aqueous barrier break with posterior sub capsular cataract progression under 80 y/o (p<0.01). Conclusions: Our results suggests that the occurrence of posterior sub capsular cataracts may be influenced by some substances in serum due to blood-aqueous barrier breaks.

Keywords: aqueous • cataract • clinical (human) or epidemiologic studies: bio