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H. Sato, R. Kawasaki, T. Yamamoto, T. Yamashita, H. Yamashita; Evaluation of Cell Damage by Measuring Vitreous Level of 8-hydroxy-2'-deoxyguanosine (8-OHdG) in Retinal Diseases . Invest. Ophthalmol. Vis. Sci. 2003;44(13):3573.
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Purpose: To evaluate the cell damage using oxidative stress to DNA, we measured the vitreous level of 8-hydroxydeoxyguanosine (8-OHdG) in eyes with retinal diseases. Methods: 8-OhdG, interleukin-6(IL-6),and vascular endothelial growth factor(VEGF) concentrations were measured by enzime-linked immunosorbentassay. NOx concentration was measured by Griess method. The vitreous samples were obtained during the vitreous surgeries from 50 eyes (22 eyes with proliferative diabetic retinopathy(PDR), 7 eyes with branch retinal vein occlusion(BRVO), 13 eyes with idiopathic macular hole, 5 eyes with epiretinal membrane, and 3 eyes with Terson syndrome), after securing the written permission from the subjects. This study has been approved by the IRB of Yamagata University School of Medicine. Results: The mean vitreal concentration of 8-OHdG in the eyes with PDR (2.33ng/mg) was significantly higher than that in the others(1.67ng/mg)(p=0.0235). The vitreal concentrations of IL-6, VEGF and NOx were significantly higher in the eyes with PDR than the others. Multivariate regression analysis has revealed that the concentration of 8-OHdG was significantly correlated with the concentration of IL-6 (p=0.0019). Conclusion: These findings indicate 8-OHdG in the vitreous was related to the cell damage in the eye.
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