May 2003
Volume 44, Issue 13
ARVO Annual Meeting Abstract  |   May 2003
Levels of Antioxidant Enzyme Genes in Conjunctival Impression Cytology Specimens from Hydrogel Contact Lenses Wearers
Author Affiliations & Notes
  • D.J. Galarreta
    Ophthalmology, IOBA, Valladolid, Spain
  • R.M. Corrales
    Ophthalmology, IOBA, Valladolid, Spain
  • J.M. Herreras
    Ophthalmology, IOBA, Valladolid, Spain
  • M.J. González
    Ophthalmology, IOBA, Valladolid, Spain
  • I. Arranz
    Ophthalmology, IOBA, Valladolid, Spain
  • V. Saez
    Ophthalmology, IOBA, Valladolid, Spain
  • A. Mayo
    Ophthalmology, IOBA, Valladolid, Spain
  • A.B. Cisneros
    Ophthalmology, IOBA, Valladolid, Spain
  • M. Calonge
    Ophthalmology, IOBA, Valladolid, Spain
  • F.J. Chaves
    Endocrinology, Hospital Clínico Universitario, Valencia, Spain
  • Footnotes
    Commercial Relationships  D.J. Galarreta, None; R.M. Corrales, None; J.M. Herreras, None; M.J. González, None; I. Arranz, None; V. Saez, None; A. Mayo, None; A.B. Cisneros, None; M. Calonge, None; F.J. Chaves, None.
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 3682. doi:
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      D.J. Galarreta, R.M. Corrales, J.M. Herreras, M.J. González, I. Arranz, V. Saez, A. Mayo, A.B. Cisneros, M. Calonge, F.J. Chaves; Levels of Antioxidant Enzyme Genes in Conjunctival Impression Cytology Specimens from Hydrogel Contact Lenses Wearers . Invest. Ophthalmol. Vis. Sci. 2003;44(13):3682.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract: : Purpose:To investigate whether hydrogel contact lens (HCL) wearing induces alterations in the expression of 5 of the most relevant antioxidant enzyme genes in the human conjunctival epithelium. Methods:Conjunctival impression cytology was performed by applying two polyethersulfone filters to the superior bulbar conjunctiva of both eyes of 8 healthy donors who never had worn contact lenses before, 6 months, and 1 year after HCL fitting, and 15 days after discontinuation. Real time PCR was performed using isolated and retrotranscribed total RNA to quantify the expression of 5 antioxidant enzyme genes: mitochondrial Mn superoxide dismutase (MnSOD), cytosolic Cu- and Zn-containing superoxide dismutase (CuZn-SOD), extracellular superoxide dismutase (ecSOD), glutathione synthetase (GSS), and glutathione reductase (GSR). The relative quantity of these mRNAs was determined by SYBR ® Green I dye detection. The housekeeping gene GAPDH was used to normalize the amount of expression levels. Chronologic variations were analyzed using Student's test and lineal regression. Results:Expression of all genes showed a tendency to diminish after 6 and 12 months of HCL wearing, although only significant for CuZn-SOD (p<0.05), and for GSS and GSR (p<0.0001). Fifteen days after discontinuing HCL wearing, ecSOD gene levels remained unaltered, whereas the other genes had a tendency to increase, being significant (p<0.05) for GSS and GSR transcripts. Conclusions: HCL wearing for 12 months decreased the expression of 3 out of the 5 antioxidant enzyme genes studied. These findings suggest that HCL wearing might decrease the defensive capacity of the normal conjunctiva against oxidative stress.

Keywords: oxidation/oxidative or free radical damage • contact lens • conjunctiva 

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