May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
ARNm Expression of TFF1 Peptide after Refractive Surgery
Author Affiliations & Notes
  • P.O. Lafontaine
    Ophthalmology, CHU-Dijon, Dijon, France
  • N. Buron
    Mort cellulaire et cancer, INSERM U-517, Dijon, France
  • M. Soulie
    Mort cellulaire et cancer, INSERM U-517, Dijon, France
  • E. Solary
    Mort cellulaire et cancer, INSERM U-517, Dijon, France
  • A.M. Bron
    Mort cellulaire et cancer, INSERM U-517, Dijon, France
  • C. Garcher
    Mort cellulaire et cancer, INSERM U-517, Dijon, France
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 3778. doi:
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      P.O. Lafontaine, N. Buron, M. Soulie, E. Solary, A.M. Bron, C. Garcher; ARNm Expression of TFF1 Peptide after Refractive Surgery . Invest. Ophthalmol. Vis. Sci. 2003;44(13):3778.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: Trefoil Factors 1 (TFF1) belong to trefoil factor family, typical secretory products of mucous epithelia. TFF are involved in gastric wound healing. They are co-expressed with mucins and act in concert with them. Our aim was to assess the kinetics of TFF1 mRNA expression during the corneal wound healing due to PRK (Photo Refractive Keratectomy). Methods: PRK was performed on 6 patients (Age: 24-38) for myopia (-1.75 to -4.25 D). Impression cytology specimens were collected before surgery and 1 hour, 1, 3, 5 and 7 days following surgery. We assessed the expression of TFF1 mRNA by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). In the same time, repeated photographs measured wound-healing areas. Results: No correlation was found between TFF1 mRNA expression and wound healing kinetic. Conclusions: TFF1 mRNA expression was increased in response to corneal ulceration. An abnormal regulation of TFF secretion could explain the delayed wound healing observed in ocular surface disease occurring after PRK. Mean of TFF1 expression (arbitrary units)  

Keywords: conjunctiva • cornea: surface mucins • refractive surgery: PRK 
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