May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Laser Scanning Confocal Microscopy Studies of Hemidesmosomal Antigens in Conjunctival Mucosa
Author Affiliations & Notes
  • J. Casiglia
    Oral medicine, Harvard School of Dental Medicine, Boston, MA, United States
  • A.R. Ahmed
    Oral medicine, Harvard School of Dental Medicine, Boston, MA, United States
  • Footnotes
    Commercial Relationships  J. Casiglia, None; A.R. Ahmed, None.
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 3804. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      J. Casiglia, A.R. Ahmed; Laser Scanning Confocal Microscopy Studies of Hemidesmosomal Antigens in Conjunctival Mucosa . Invest. Ophthalmol. Vis. Sci. 2003;44(13):3804.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Abstract: : Purpose: Samples of human conjunctiva were obtained from cataract surgery on patients without any inflammatory ocular disease. Methods: Samples were immediately fixed, and subsequently embedded in OCT, and sectioned into 8 micron sections. The samples were then placed on slides and stained with primary antibodies to antigens present in hemidesmosomal structures. Primary antibodies were diluted at a concentration of 1:500 and incubated overnight at 4 C. Secondary (flurochrome-conjugated) antibodies were then added, and the process repeated for double or triple-staining as necessary. Antigens evaluated included BP180 (BPAg2), alpha6Beta4 integrin, collagen VII, and laminin 5. Results: Fluorescent staining demonstrated colocalization of subunits of alpha6beta4 integrin, as well as localized staining of these subunits with the BP180 antigen. Collagen VII also stained strongly beneath the basement membrane. Interestingly, laminin 5 could not be visualized—at least not to the same extent as demonstrated in similar studies with normal human oral mucosa and normal human epidermis. Conclusions: This study is the initial step in the use of confocal microscopy to structurally describe the relationships of these critical molecules.

Keywords: cell-cell communication • cell adhesions/cell junctions • conjunctiva 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×