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M. Watanabe, W. Yano, S. Kondo, A. Ohhira, Y. Hattori, T. Nishida; Human Annexin A5 Promotes the Migration of Rabbit Corneal Epithelial Cells . Invest. Ophthalmol. Vis. Sci. 2003;44(13):3823.
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Purpose: To investigate the effects of annexin A5, a calcium-dependent phospholipid binding protein, on the rabbit corneal epithelial wound healing in vitro and in vivo. Methods:In vitro study, confluent monolayers of primary cultured rabbit corneal epithelial cells were wounded with a scraper and subsequently cultured in the presence of recombinant human annexin A5. The distance of cell migration into the denuded area was measured after 24 hours. In vivo corneal wound closure model, corneal epithelium was removed by iodine vapor in rabbit. Human annexin A5 eye drops were administered, and the size of the epithelial defect was measured. The wound healing rate was calculated by linear regression analysis. Results:Human annexin A5 promotes the migration of rabbit corneal epithelial cells in vitro. In vivo corneal wound closure model, the wound healing rate of eyes administered with human annexin A5 was significantly higher than the rate of control eye administered with phosphated buffered saline. Conclusions:These results suggested that human annexin A5 promotes corneal wound healing.
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