May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Expression of Receptors for Advanced Glycosylation End Products on Bovine Retinal Capillary Pericytes in vitro
Author Affiliations & Notes
  • X. Xu
    Ophthalmology, Shanghai First Peoples Hosp, Shanghai, China
  • X. Xia
    Ophthalmology, Shanghai First Peoples Hosp, Shanghai, China
  • Footnotes
    Commercial Relationships  X. Xu, None; X. Xia, None.
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 3883. doi:
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      X. Xu, X. Xia; Expression of Receptors for Advanced Glycosylation End Products on Bovine Retinal Capillary Pericytes in vitro . Invest. Ophthalmol. Vis. Sci. 2003;44(13):3883.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To survey the expression of receptors for advanced glycosylation end products on bovine retinal pericytes. Methods: Proteins that have been modified by long-term expose to glucose accumulate advanced glycosylation end products (AGEs), which are found in the plasma and accumulate in the tissues during aging and at an accelerated rate in diabetes. A novel integral membrane protein, termed receptor for AGE (RAGE), forms a central part of the cell surface binding site for AGEs. In these studies, we have examined the interaction of AGE-BSA with bovine retinal capillary pericytes (BRP). Using polyconal antibody raised to synthetic peptide that corresponds to amino acids 42-59 of the human receptor for advanced glycated end products and reverse transcriptase-polymerase chain reaction of RAGE mRNA to detect the expressiong of RAGE. Results: Immunostaining showed RAGE in the retinal pericytes. Consistent with these data, RAGE mRNA were identified through reverse transcriptase-polymerase chain reaction. Conclusions: These results indicate that RAGE is present in retinal pericytes and suggest that AGEs taken up through RAGE on retinal pericytes might play a role in influencing the growth of retinal pericytes.

Keywords: diabetic retinopathy • immunohistochemistry • vascular cells 
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