May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Expression of Inwardly Rectifying K+ (Kir) Channels in Retinal Bipolar Cells
Y. Ma; J. Cui; H. Hu; Z. Pan
Author Affiliations & Notes
  • Y. Ma
    Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, MI, United States
  • J. Cui
    Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, MI, United States
  • H. Hu
    Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, MI, United States
  • Z. Pan
    Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, MI, United States
  • Footnotes
    Commercial Relationships  Y. Ma, None; J. Cui, None; H. Hu, None; Z. Pan, None.
  • Footnotes
    Support  NIH RO1EY12180 , Vision Core Grant EY04068
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 4152. doi:
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      Y. Ma, J. Cui, H. Hu, Z. Pan; Expression of Inwardly Rectifying K+ (Kir) Channels in Retinal Bipolar Cells . Invest. Ophthalmol. Vis. Sci. 2003;44(13):4152.

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Abstract

Abstract: : Purpose: Retinal bipolar cells have been reported to express hyperpolarization-activated cationic currents (Ih). We have previously observed a differential expression of Ih among different retinal bipolar cells in the rat (Hu & Pan, 2000 ARVO abstract). In this study, we report that retinal bipolar cells also express inwardly rectifying K+ current (IKir). Methods: Whole-cell patch-clamp recordings were performed in acutely isolated retinal bipolar cells and in bipolar cells in retinal slices from the rat. Results: Hyperpolarization steps evoked two types of inwardly rectifying currents. One was the previously reported Ih, which showed slow time-dependent activation. Consistently, Ih was carried by both Na+ and K+ ions and blocked by ZD7288, a selective blocker of Ih. The second current displayed rapid activation and no apparent inactivation. The current was carried by K+ ions. The activation threshold and the current-voltage relation shifted with a change in extracellular K+, a classical property of Kir channels. Furthermore, the current was found to be highly sensitive to Ba2+, a blocker of Kir channels. Retinal bipolar cells of different groups were found to differentially express IKir and Ih. A subset of CBCs showed only IKir. RBCs displayed mainly Ih. Conclusions: This is the first report of the expression of Kir channels in retinal bipolar cells. The differential expression of IKir and Ih in retinal bipolar cells may play a role in their diverse physiological functions.

Keywords: ion channels • bipolar cells • retina: distal(photoreceptors, horizontal cell 
© 2003, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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