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P. Ahuja, S.N. Archer, T. van Veen, M. von Schantz; Neuroleukin/AMF Is Present Around Cones and in Various Spatially Restricted Cell Types of the Mouse Retina . Invest. Ophthalmol. Vis. Sci. 2003;44(13):4182.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: During an attempt to identify genes differentially expressed in rd (retinal degeneration) retina compared to wildtype we discovered higher expression of neuroleukin in the former. Neuroleukin is also known as phosphoglucose isomerase and extracellularly as autocrine motility factor (AMF). The neuroleukin/AMF receptor distribution has been examined in brain, but little is known regarding its presence in retina. We therefore undertook a developmental study of neuroleukin expression in mouse retina. Methods: C3H +/+ and rd/rd mice from postnatal day 2 (PN2), PN7, PN14, PN21, PN28 ages were sacrificed. The eye was enucleated and the posterior eyecup fixed by immersion in buffered paraformaldehyde. 8µm frozen sections were cut. The sections were incubated with an anti-neuroleukin antibody (Dr. A. Raz, Wayne State University) alone or combinations of this and another antibody directed against blue-cone visual pigment (OS-2; Dr. A. Szel, Semmelweis University). Standard immunohistochemical procedures were followed. Results: At PN2 in both genotypes clear neuroleukin immunolabeling was present in the cells of the relatively well organized GCL, as well as in 3 to 4 rows of cells adjacent to the developing IPL. In addition, scattered, mostly elongated, cells in outer regions of the neuroblast layer showed neuroleukin immunoreactivity. The staining intensity in the GCL was much greater than that in the neuroblast layer. At PN7 in both genotypes the stained cells in the developing INL were more condensed and positioned adjacent to the IPL, in which short labeled neurites were also seen. The staining intensity of cells in the INL had increased. At PN14 in both genotypes the adult neuroleukin staining pattern in the inner retina is reached with intensely labeled elements in the GCL and weaker labeled cells in the INL adjacent to both the IPL and OPL. From PN21 onwards clear labeling is also observed in the photoreceptor segment layer. Double labeling showed neuroleukin presence around all cones. Conclusions: The main observations are: (1) neuroleukin is present in a spatially restricted cell population of the early postnatal retina. We assume that this phenomenon provides some functionality and we will test if neuroleukin-expressing cells in the inner retina are more or less sensitive to experimental glaucoma. (2) neuroleukin is present around all cones. Since its extracellular distribution is linked to cell motility we hypothesize that this observation might indicate retinomotor movement capacity of mammalian cones.
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