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C.P. Howard, Y. Wang, J. Harmon, J. Hartke, D.P. Edward, M.B. Wax; Immunohistochemical Characterization of Matrix Metalloproteinase-13 Expression in Ocular Tissues . Invest. Ophthalmol. Vis. Sci. 2003;44(13):4384.
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Purpose: Retinal pigmented epithelial cells and/or non-pigmented epithelial cells have been reported to manufacture most of the extracellular matrix components associated with Bruch's membrane and the vitreous of the eye. Homeostasis in normal systems is maintained by equilibrium between synthesis and degradation. We sought to study the ocular localization of collagenase-3 (MMP-13), which preferentially cleaves type II collagen, a major component of the vitreous. Methods: The expression of matrix metalloproteinase-13 (MMP-13, collagenase-3) was examined by immunohistochemistry in rat and mouse ocular tissue sections that were fixed in 10% formalin using a commercial antibody raised against human MMP-13 that is known to cross-react with both mouse and rat homologues. Tissue sections from eyes of lipopolysaccharide (LPS) treated mouse eyes were also examined to see whether MMP-13 expression was induced in the retina and ciliary body, as has been reported in the cornea, associated with inflammation such as occurs with pathogen infection. Results: Constitutive expression of MMP-13 was observed in the ciliary body in all the eyes examined. The MMP-13 was localized between the non-pigmented epithelial cells and pigmented epithelial cells along the apical face of the plasma membranes. Striking MMP-13 expression was observed in ciliary body from the LPS treated mouse, but the localization was unchanged. Conclusions: The epithelial cells that line the outer perimeter of the ciliary body constitutively express MMP-13. LPS treatment augments the expression, suggesting that MMP-13 upregulation can occur in inflammatory states. We suggest that upregulation of ciliary epithelial MMP may modulate fluid flow in this tissue, such as may occur in hypotony associated with uveitis, or with topical treatment of PG analogues.
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