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T. Akaishi, T. Matsugi, Y. Takagi, N. Ishida, H. Hara, K. Kashiwagi; Effects of Combined Treatment with Bunazosin Hydrochloride and Latanoprost on Intraocular Pressure in Monkeys . Invest. Ophthalmol. Vis. Sci. 2003;44(13):4388.
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Purpose: Bunazosin hydrochloride, a selective α1 adrenergic-receptor antagonist, reduces intraocular pressure (IOP) in glaucoma patients. This study evaluated the possible additive ocular hypotensive effects of bunazosin hydrochloride and latanoprost in ocular normotensive monkeys, and examined the effects of these two compounds on matrix metalloproteinase (MMP) activities in monkey cultured ciliary muscle cells. Methods: IOP was measured before and after instillation of bunazosin hydrochloride (0.01%) and latanoprost (0.005%) alone or in combination in ocular normotensive monkeys. Monkey ciliary muscle cells were grown to confluence in culture, then exposed to bunazosin hydrochloride (10-7 - 10-5 M) or latanoprost (10-6 M) for 72 hr. The activities of MMP-2, -3 and -9 in the medium were examined using gelatin and casein zymography. Results: The peak and maximal reduction in IOP of the combination of bunazosin hydrochloride and latanoprost were greater than those of bunazosin hydrochloride and latanoprost alone, respectively. Treatment with latanoprost increased MMP-2, -3 and -9 activities in the culture medium of ciliary muscle cells, but bunazosin hydrochloride did not. Conclusions: In ocular normotensive monkey, the combination of bunazosin hydrochloride and latanoprost has an effect on IOP that is greater than that of either drug given alone. Bunazosin hydrochloride reduced IOP without increasing MMP activities in ciliary muscle cells.
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