May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Transscleral Delivery of an Anti-VEGF Aptamer in a Rabbit Model
K.G. Carrasquillo; S. Ray; I.K. Rigas; P. Calias; J.W. Miller; E.S. Gragoudas; A.P. Adamis
Author Affiliations & Notes
  • K.G. Carrasquillo
    Chemistry/Drug Delivery, Eyetech Pharmaceuticals, Woburn, MA, United States
  • S. Ray
    Angiogenesis Laboratory and Retina Service, Massachusetts Eye and Ear Infirmary, Boston, MA, United States
  • I.K. Rigas
    Angiogenesis Laboratory and Retina Service, Massachusetts Eye and Ear Infirmary, Boston, MA, United States
  • P. Calias
    Angiogenesis Laboratory and Retina Service, Massachusetts Eye and Ear Infirmary, Boston, MA, United States
  • J.W. Miller
    Angiogenesis Laboratory and Retina Service, Massachusetts Eye and Ear Infirmary, Boston, MA, United States
  • E.S. Gragoudas
    Angiogenesis Laboratory and Retina Service, Massachusetts Eye and Ear Infirmary, Boston, MA, United States
  • A.P. Adamis
    Angiogenesis Laboratory and Retina Service, Massachusetts Eye and Ear Infirmary, Boston, MA, United States
  • Footnotes
    Commercial Relationships  K.G. Carrasquillo, Eyetech Pharmaceuticals E; S. Ray, None; I.K. Rigas, None; P. Calias, Eyetech Pharmaceuticals E; J.W. Miller, Eyetech Pharmaceuticals P; E.S. Gragoudas, Eyetech Pharmaceuticals P; A.P. Adamis, Eyetech Pharmaceuticals E.
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 4442. doi:
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      K.G. Carrasquillo, S. Ray, I.K. Rigas, P. Calias, J.W. Miller, E.S. Gragoudas, A.P. Adamis; Transscleral Delivery of an Anti-VEGF Aptamer in a Rabbit Model . Invest. Ophthalmol. Vis. Sci. 2003;44(13):4442.

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Abstract

Abstract: : Purpose: To establish methods for the controlled delivery of bioactive anti-VEGF aptamer via the transscleral route. Methods: The anti-VEGF aptamer was encapsulated into PLGA (50/50) polymer microspheres and packed into a PDMS delivery device. Devices containing drug-loaded microspheres or blank microspheres, right and left eyes respectively, were placed on the temporal sclera of Dutch belted rabbits. One and two weeks following device placement, aptamer delivery and bioactivity were assessed via the inhibition of VEGF-induced blood-retinal barrier breakdown. Blood-retinal barrier breakdown was induced through the intravitreal injection of VEGF (1 µg/ml) and was quantified via the Evans blue method. Results: Blood-retinal barrier breakdown was potently induced by VEGF. Placement of PDMS devices loaded VEGF aptamer containing microspheres inhibited blood-retinal barrier. The inhibition was effective 1 and 2 weeks after device placement (P<0.05). Conclusions: In vivo transcleral delivery of a bioactive molecule has been demonstrated utilizing an anti-VEGF aptamer. By combining microsphere encapsulation with transcleral delivery, VEGF-induced retinal vascular permeability was decreased over a two-week period. These results represent a new delivery approach for anti-VEGF aptamers.

Keywords: neovascularization • receptors: pharmacology/physiology • retinal neovascularization 
© 2003, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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