May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Effects of Intravitreal Indocyanine Green Injection in Rabbits
Author Affiliations & Notes
  • M. Maia
    Ophthalmology, Paulista School of Medicine, Sao Paulo, Brazil
  • E. Margalit
    Ophthalmology, Wilmer Eye Institute, Baltimore, MD, United States
  • R. Grebe
    Ophthalmology, Wilmer Eye Institute, Baltimore, MD, United States
  • E. de Juan
    Ophthalmology, Doheny Eye Institute, Los Angeles, CA, United States
  • K.G. Au Eong
    Ophthalmology, Doheny Eye Institute, Los Angeles, CA, United States
  • M.E. Farah
    Ophthalmology, Doheny Eye Institute, Los Angeles, CA, United States
  • M.O. Tso
    Ophthalmology, Doheny Eye Institute, Los Angeles, CA, United States
  • J. Weiland
    Ophthalmology, Doheny Eye Institute, Los Angeles, CA, United States
  • G. Fujii
    Ophthalmology, Doheny Eye Institute, Los Angeles, CA, United States
  • M.S. Humayun
    Ophthalmology, Doheny Eye Institute, Los Angeles, CA, United States
  • Footnotes
    Commercial Relationships  M. Maia, None; E. Margalit, None; R. Grebe, None; E. de Juan, None; K.G. Au Eong, None; M.E. Farah, None; M.O.M. Tso, None; J. Weiland, None; G. Fujii, None; M.S. Humayun, None.
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 4445. doi:
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      M. Maia, E. Margalit, R. Grebe, E. de Juan, K.G. Au Eong, M.E. Farah, M.O. Tso, J. Weiland, G. Fujii, M.S. Humayun; Effects of Intravitreal Indocyanine Green Injection in Rabbits . Invest. Ophthalmol. Vis. Sci. 2003;44(13):4445.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose:To report clinical, electrophysiological and histological findings of different concentrations of indocyanine green (ICG) injected into the vitreous cavity of rabbit eyes.Methods:Forty-two rabbits had intravitreal injection of 0.1ml of ICG in three different concentrations: 0.5mg/ml (250mOsm), 5mg/ml (270mOsm) and 25mg/ml (170 mOsm). The opposite eyes were injected with 0.1ml of balanced salt solution (BSS). Biomicroscopy, ophthalmoscopy, electroretinography, fluorescein angiography and histological evaluation were performed 2 hours, 4 hours, 24 hours, 7 days and 28 days after ICG injection.Results:Eyes injected with 0.5mg/ml of ICG had a delay in the B-wave latency on the first day after injection (P<0.05). Eyes injected with 5mg/ml of ICG had a delay in the B wave latency and a decrease in B-wave amplitude on the first and seventh days after injection (P<0.01); a delay in the A wave latency on the first day after injection (p<0.05) was also observed. Eyes injected with 25mg/ml of ICG had both B and A-waves amplitude and latency abnormalities during the entire follow up (P<0.01). Histology demonstrated a direct relation of increasing amount of retinal edema proportional to the progressive ICG concentrations.Conclusions:Intravitreal injection of ICG in rabbit eyes may impair retinal function and morphology proportionally to the progressive ICG dosages.

Keywords: animal model • pathology: experimental • vitreoretinal surgery 
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