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R. Mancini, L.E. Goldstein, L.T. Chylack Jr; Lens Epithelial-Derived Growth Factor (LEDGF) is Expressed in Neurons Within the Mammalian Central Nervous System . Invest. Ophthalmol. Vis. Sci. 2003;44(13):4509.
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Purpose: Lens-epithelium-derived growth factor (LEDGF) is 530 amino acid transcription factor with potent antioxidative and cytoprotective properties. LEDGF, also known as p75, shares homology with the p75 splice variant p52, heptoma-derived growth factor (HDGF), and HDGF-related proteins 1 and 2. The LEDGF gene has been sequenced and assigned (9p22.2). LEDGF may play a central role in lens epithelial cell differentiation and potently promotes survival of these cells in response to thermal, oxidative and other stresses (Singh, D.P. et al. IOVS  40:1444). Since common degenerative disorders of the brain (e.g., Alzheimer’s disease) involve oxidative damage and associated cytotoxicity, we hypothesized that LEDGF may be expressed in neurons. Here we demonstrate and characterize the expression of LEDGF within neurons within the mammalian central nervous system.Methods: We examined guinea pig and mouse primary neuronal cell cultures, as well as mouse and human brain specimen, using western blot, immunohistofluorescence, and mass spectrometry to confirm the expression, localization, and distribution of LEDGF within neurons of the central nervous system. Results: We have conclusively demonstrated that neurons and some glial elements within the mammalian central nervous system express LEDGF.Conclusions: LEDGF is expressed in neurons within the mammalial central nervous system. This transcription factor may play a role in neuronal differentiation and responses to oxidative insult associated with Alzheimer's disease.
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