May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
P53-Mediated Apoptosis in Human RPE Cells After Low-Dose Radiation
Y. Jiang; M.F. Escaño; S. Kusuhara; S. Fujii; R. Sasaki; A. Negi
Author Affiliations & Notes
  • Y. Jiang
    Ophthalmology, Kobe Univ Grad Sch Med, Chuo-Ku, Japan
  • M.F. Escaño
    Ophthalmology, Kobe Univ Grad Sch Med, Chuo-Ku, Japan
  • S. Kusuhara
    Ophthalmology, Kobe Univ Grad Sch Med, Chuo-Ku, Japan
  • S. Fujii
    Ophthalmology, Kobe Univ Grad Sch Med, Chuo-Ku, Japan
  • R. Sasaki
    Radiology, Kobe Univ Grad Sch Med, Chuo-Ku, Japan
  • A. Negi
    Radiology, Kobe Univ Grad Sch Med, Chuo-Ku, Japan
  • Footnotes
    Commercial Relationships  Y. Jiang, None; M.F.T. Escaño, None; S. Kusuhara, None; S. Fujii, None; R. Sasaki, None; A. Negi, None.
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 4546. doi:
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      Y. Jiang, M.F. Escaño, S. Kusuhara, S. Fujii, R. Sasaki, A. Negi; P53-Mediated Apoptosis in Human RPE Cells After Low-Dose Radiation . Invest. Ophthalmol. Vis. Sci. 2003;44(13):4546.

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Abstract

Abstract: : Purpose: To investigate the molecular basis for cell growth inhibition or apoptosis in human RPE cells after low-dose radiation. Methods: Cell survival assay, TdT-mediated dUTP-biotin nick-end labeling (TUNEL) assay were performed on post-radiated RPE cell cultures at different periods. Levels of p53, p21, Bax, Fas-FasL were evaluated by semiquantitative reverse transcriptional polymerase chain reaction (RT-PCR). p53 was analyzed by PCR single-strand conformation polymorphism (PCR-SSCP) mutational assay and Western blot. Results: ARPE-19 cell viability decreased with increasing doses of radiation. Apoptosis was detected after 6 hours of radiation. Increase in p53, p21 and Bax mRNA expression by semiquantitative RT-PCR analysis was greatest in the 2-hour post-radiation samples. Transcription levels of Fas and FasL remained unchanged. Protein levels of p53 were increased at the 4- and 6-hour post-radiation samples. Conclusions: Low-dose radiation induces a p53-dependent apoptotic mechanism in RPE cells in vitro.

Keywords: retinal pigment epithelium • radiation therapy • apoptosis/cell death 
© 2003, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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