May 2003
Volume 44, Issue 13
ARVO Annual Meeting Abstract  |   May 2003
Prevention of Apoptosis in an RPE Carcinoma Cell Line by Bile Acids
Author Affiliations & Notes
  • V.T. Do
    Ophthalmology, Emory University, Atlanta, GA, United States
  • J.M. Nickerson
    Ophthalmology, Emory University, Atlanta, GA, United States
  • J.H. Boatright
    Ophthalmology, Emory University, Atlanta, GA, United States
  • Footnotes
    Commercial Relationships  V.T. Do, None; J.M. Nickerson, None; J.H. Boatright, None.
  • Footnotes
    Support  NIH Grant R01 EY12514, R03 EY13986, P30 EY06360, FFB, RPB
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 4550. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      V.T. Do, J.M. Nickerson, J.H. Boatright; Prevention of Apoptosis in an RPE Carcinoma Cell Line by Bile Acids . Invest. Ophthalmol. Vis. Sci. 2003;44(13):4550.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Abstract: : Purpose: Recent studies show evidence that retinal pigment epithelial cells (RPE) undergo apoptosis in human age-related macular degeration (AMD) and retinal dystrophies. The inhibition of apoptosis may aid in the treatment of such diseases. Tauroursodeoxycholic acid (TUDCA), a bile acid, prevents apoptosis of neuronal cells in animal models of Huntington's Disease. We sought to determine whether TUDCA also prevents apoptosis in RPE-like cells. Methods: Cells derived from RPE carcinomas were pre-treated for 20 hours with 200 uM of TUDCA. The media was removed and replaced with either serum-free or full media containing 200 uM of TUDCA. Apoptosis was induced through serum starvation and/or oxidative damage by incubation with 300 uM of tert-butylhydroperoxide (tBH). After 24 hours, cells were harvested for analysis. Apoptosis was assessed using a modified TUNEL assay. DNA was extracted using a Qiagen kit and electrophoresed on a 1.8% agarose gel to determine if DNA laddering occurred. Results: Cells that were treated with TUDCA had fewer TUNEL-positive cells than those that were not. DNA laddering was apparent in samples from cultures undergoing oxidative stress and starvation. Cells that were incubated with TUDCA had diminished or non-existent DNA laddering. Conclusions: Our DNA laddering and TUNEL analyses show that TUDCA is effective in stopping or slowing induced apoptosis in RPE-like cells. Bile acids offer advantages as potential therapeutic agents for AMD and retinal dystrophies as they are nontoxic, FDA approved, easy to administer, and inexpensive.

Keywords: retinal pigment epithelium • cell death/apoptosis • pharmacology 

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.