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Y. Li, Y. Zhuo, M. Zhou; Cytoskeletal Protein 4.1 G Binds to Potassium Channel Kv1.3 in the Rat Retina . Invest. Ophthalmol. Vis. Sci. 2003;44(13):4580.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To study the possible interacting partner of the potassium channel, Kv1.3 in the rat retina. Methods: Rat retina fraction were extracted, the cell extracts was incubated with the anti-Kv1.3 antibody or Anti-4.1G antibody for co-immunoprecipitation assay, the bound proteins were examined by western blot with anti-4.1G or anti-kv1.3 antibody, the signal was detected using ECL; The localization of Kv1.3 and 4.1 G were detected with immunostaining. Results: With the Co-immunoprecipatation assay, with the anti-4.1G to immunoprecipated the kv1.3/4.1G heteromers, the Kv1.3 could be shown with western blot, and vice versa. Immunohistochemical studies showed that 4.1G is widespread present in the retina, Kv1.3 mainly distribution in the RGC and RPE. Our results show that co-distribution of 4.1 G and potassium channel protein Kv1.3 in the rat retina suggests a potential interaction at the retina. Conclusion: 4.1G may act as a key player in the rat retina signal transduction pathways. It may help deliver, mobilize and localize potassium channel to cell membrane to exert their function.
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