Peritoneal Exudate Cells Cultured with Calcitonin Gene-Related Peptide can Suppress Murine Experimental Autoimmune Uveoretinitis via IL-10

Abstract

Abstract: : Purpose: To investigate the role of the suppression in murine experimental autoimmune uveoretinitis (EAU) by peritoneal exudate cells (PEC) cultured with calcitonin gene-related peptide (CGRP). Methods: PEC derived from C57BL/6 mice were cultured with CGRP and residues 1-20 of human interphotoreceptor retinoid binding protein (IRBP). EAU was induced by immunization with residues 1-20 of IRBP in Freund’s adjuvant and pertussis toxin in C57BL/6 mice. At the same time, IRBP-peptide immunized mice were injected the PEC cultured with CGRP and IRBP-peptide intravenously. On day 19 after immunization, peptide-specific delayed hypersensitivity (DH) were measured. On day 21 after immunization, animals were sacrificed, and assess the extent of EAU pathologically. Some studies were injected anti-IL-10 antibody into EAU mice treated with CGRP-cultured PEC intraperitonealy, or used IL-10 KO mice as source of PEC. Results: EAU and antigen-specific DH was suppressed by injection of PEC cultured with CGRP (100ng/ml) and human IRBP-peptide. However, human IRBP-peptide mediated EAU was not suppressed by injection of PEC cultured with CGRP and BSA. Furthermore, EAU and antigen-specific DH was not suppressed by injection of PEC cultured with CGRP and IRBP-peptide when mice were injected anti-IL-10 antibody intraperitonealy, or by injection of IL-10 KO mice derived PEC cultured with CGRP and IRBP-peptide. Conclusions: PEC cultured with CGRP could suppress murine EAU in an antigen-specific manner, and IL-10 secreted from PEC might play an important role on the CGRP mediated suppression of murine EAU.

Keywords: uveitis-clinical/animal model • immunomodulation/immunoregulation • immune tolerance/privilege