May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Asymmetric Systemic Lymphadenopathy and Intraocular Inflammation in Autoimmune Anterior Uveitis
Author Affiliations & Notes
  • H. Nishihori
    Ophthalmology and Visual Sciences, Kentucky Lions Eye Center, University of Louisville, Louisville, KY, United States
  • J.M. Cruz
    Ophthalmology and Visual Sciences, Kentucky Lions Eye Center, University of Louisville, Louisville, KY, United States
  • J. Sohn
    Ophthalmology and Visual Sciences, Kentucky Lions Eye Center, University of Louisville, Louisville, KY, United States
  • Y. Wang
    Ophthalmology and Visual Sciences, Kentucky Lions Eye Center, University of Louisville, Louisville, KY, United States
  • H.J. Kaplan
    Ophthalmology and Visual Sciences, Kentucky Lions Eye Center, University of Louisville, Louisville, KY, United States
  • N.S. Bora
    Ophthalmology and Visual Sciences, Kentucky Lions Eye Center, University of Louisville, Louisville, KY, United States
  • Footnotes
    Commercial Relationships  H. Nishihori, None; J.M. Cruz, None; J. Sohn, None; Y. Wang, None; H.J. Kaplan, None; N.S. Bora, None.
  • Footnotes
    Support  NIH EY13094, RPB, Inc, NY and Commonwealth of KY Research Challenge Trust Fund
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 4592. doi:
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    • Get Citation

      H. Nishihori, J.M. Cruz, J. Sohn, Y. Wang, H.J. Kaplan, N.S. Bora; Asymmetric Systemic Lymphadenopathy and Intraocular Inflammation in Autoimmune Anterior Uveitis . Invest. Ophthalmol. Vis. Sci. 2003;44(13):4592.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To investigate lymphoycte cell trafficking in experimental autoimmune anterior uveitis (EAAU). Methods: EAAU was induced in Lewis rats by the foot-pad injection of melanin associated antigen (MAA). Lymph node (LN) weight and total lymphocyte cell count was determined in both ipsilateral (to the site of injection) and contralateral lymph nodes at four different time points – before the onset of uveitis (day 10), upon the onset (day 14), at the peak (day 18) and after resolution (day 30). Harvested lymphocytes were also analyzed for cell surface markers for T cells (both CD4 and CD8) and macrophages. The results for naïve and MAA-injected animals was compared. Results: As expected, before the onset of uveitis (i.e. day 10) and onwards there was a significant increase in the weight and cell number in the draining ipsilateral lymph nodes (popliteal and inguinal) compared to the contralateral nodes. Increased numbers of CD4+ T cells, CD8+ T cells and macrophages were observed in the ipsilatreral lymph nodes at all time points studied – CD4+ T cells being the predominant cell population. Interestingly, at various time points the ispsilateral axillary (days 10 and 30) and cardiac (day 18) lymph nodes showed a significant increase in the both weight and cell number compared to the contralateral nodes with the same mononuclear cell profile. No difference was observed in the cervical lymph nodes at any time point. The anterior uveitis was present in both eyes almost simultaneously although in 10% of rats intraocular inflammation developed in the OS 24 hours before OD as detected by slit-lamp biomicroscopy. Conclusions: EAAU induced by the injection of MAA in the foot-pad results in a bilateral acute anterior uveitis. Surprisingly, we observed an asymmetric lymphadenopathy in susceptible hosts. Sensitive methods of non-invasively tracking lymphocyte cell migration and lymph node size may reveal similar changes in man. The pathophysiologic signficance of this observation is not known at the present time.

Keywords: autoimmune disease • uveitis-clinical/animal model • pathology: experimental 
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