May 2003
Volume 44, Issue 13
ARVO Annual Meeting Abstract  |   May 2003
Altered Distribution of Polymorphisms in the Promoter Gene for IL-10 in Patients with Behcet's Disease but not Those with Idiopathic Retinal Vasculitis
Author Affiliations & Notes
  • Y. Chen
    Rayne Institute Ophthalmology, St Thomas Hospital/King's College London, London, United Kingdom
  • R. Vaughan
    Tissue Typing, Guy's Hospital/King's College London, London,
  • E. Kondeatis
    Tissue Typing, Guy's Hospital, London,
  • E.M. Graham
    Ophthalmology, St Thomas Hospital, London,
  • F. Fortune
    Oral Medicine, University of Leeds, Leeds,
  • G.R. Wallace
    Rayne Inst Ophthalmology, St Thomas Hospital /King's College London, London,
  • M.R. Stanford
    Rayne Institute Ophthalmology, St Thomas Hospial/King's College London, London,
  • Footnotes
    Commercial Relationships  Y. Chen, None; R. Vaughan, None; E. Kondeatis, None; E.M. Graham, None; F. Fortune, None; G.R. Wallace, None; M.R. Stanford, None.
  • Footnotes
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Investigative Ophthalmology & Visual Science May 2003, Vol.44, 4598. doi:
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      Y. Chen, R. Vaughan, E. Kondeatis, E.M. Graham, F. Fortune, G.R. Wallace, M.R. Stanford; Altered Distribution of Polymorphisms in the Promoter Gene for IL-10 in Patients with Behcet's Disease but not Those with Idiopathic Retinal Vasculitis . Invest. Ophthalmol. Vis. Sci. 2003;44(13):4598.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract: : Purpose: Polymorphisms in the upstream region of the anti-inflammatory cytokine IL-10 have been associated with alterations in its transcriptional activity. We studied the prevalence of 3 IL-10 promoter region polymorphisms in patients with Behcet’s disease and idiopathic retinal vasculitis. Methods: DNA was prepared from 74 patients with Behcet’s disease, 160 patients with retinal vasculitis and 100 controls. Polymorphisms in the IL-10 gene region at positions –592, -819 and -1082 were detected by a multiplex SSP-PCR assay. Associations with disease were calculated by both allelic frequency and haplotype analysis. Results: Haplotype and allelic analysis revealed no significant differences in patients with retinal vasculitis compared to controls. Comparisons of the allelic frequencies showed a higher prevalence of the –1082 A allele and a lower prevalence of the –1082 G allele in patients with Behcet’s disease (pc=0.007; OR for A=2.09, 95%CI 1.37-3.21. OR for G =0.59, 95%CI 0.38-0.93). In patients with Behcet’s disease there was a higher prevalence of the ATA compared to the ACC and GCC haplotypes (pc<0.008; OR=2.79, 95% CI 1.72-4.52). This was particularly true in patients with two copy haplotypes (pc=0.024; OR=5.14 95%CI 1.67-15.62). Conversely, the GCC haplotype was under-represented in patients (pc=0.032;OR=0.38, 95%CI 0.20-0.75) No patients with ocular involvement had the two copy GCC haplotype (pc<0.001; OR=0.0016 95%CI 0.0009-0.291) Conclusion: We describe an abnormal distribution of polymorphism in haplotypes of the IL-10 promoter region in patients with Behcet’s disease, especially those with eye involvement. This altered distribution may partly explain the immunological dysfunction observed in this disease.

Keywords: genetics • immunomodulation/immunoregulation • uveitis-clinical/animal model 

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