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A. Sanchez-Navarro, A. Climent, R. Suárez, J.C. Cancino, R. López-Revilla, M. Reyes-López; Detection of the Herpes Simplex Virus-1 Genome and Capsid Antigens in Ocular Samples from Patients with Herpetic Keratitis . Invest. Ophthalmol. Vis. Sci. 2003;44(13):4631.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To compare the detection of the Herpes Simplex virus-1 (HSV-1) genome and capsid antigens in ocular samples from patients with epithelial and stromal herpetic keratitis. Methods: The polymerase chain reaction (PCR) and ELISA (Herpchek) were respectively used to detect the HSV-1 genome (DNA) and capsid antigens in corneal scrapings and tears from 8 patients with epithelial keratitis and 13 patients with inactive herpetic stromal keratitis. Results: In the epithelial keratitis samples viral DNA and antigens were detected in 6 (75%) corneal scrapings, whereas in tears viral DNA was found in 7 (88%) samples and viral antigens were not detected at all; Herpchek positive cases were also PCR positive. All stromal herpetic keratitis samples were negative both by PCR and Herpchek. Conclusions: In epithelial herpetic keratitis the presence of HSV-1 DNA should be confirmed using PCR in tear or corneal samples given its superior sensitivity to viral antigen detection using Herpchek. Since HSV-1 DNA and antigens cannot be detected in inactive stromal keratitis cases, we will explore the active entity in the future.
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