May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Detection of the Herpes Simplex Virus-1 Genome and Capsid Antigens in Ocular Samples from Patients with Herpetic Keratitis
A. Sanchez-Navarro; A. Climent; R. Suárez; J.C. Cancino; R. López-Revilla; M. Reyes-López
Author Affiliations & Notes
  • A. Sanchez-Navarro
    Departmento of Molecular Microbiology, Instituto de Oftalmologia Conde de Valenciana, Mexico D.F., Mexico
  • A. Climent
    Departmento of Molecular Microbiology, Instituto de Oftalmologia Conde de Valenciana, Mexico D.F., Mexico
  • R. Suárez
    Department of Córnea, Instituto de Oftalmologia Conde de Valenciana, Mexico D.F., Mexico
  • J.C. Cancino
    Department of Córnea, Instituto de Oftalmologia Conde de Valenciana, Mexico D.F., Mexico
  • R. López-Revilla
    Department of Cell Biology, CINVESTAV-IPN, Mexico D.F., Mexico
  • M. Reyes-López
    Department of Cell Biology, CINVESTAV-IPN, Mexico D.F., Mexico
  • Footnotes
    Commercial Relationships  A. Sanchez-Navarro, None; A. Climent, None; R. Suárez, None; J.C. Cancino, None; R. López-Revilla, None; M. Reyes-López, None.
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 4631. doi:
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      A. Sanchez-Navarro, A. Climent, R. Suárez, J.C. Cancino, R. López-Revilla, M. Reyes-López; Detection of the Herpes Simplex Virus-1 Genome and Capsid Antigens in Ocular Samples from Patients with Herpetic Keratitis . Invest. Ophthalmol. Vis. Sci. 2003;44(13):4631.

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Abstract

Abstract: : Purpose: To compare the detection of the Herpes Simplex virus-1 (HSV-1) genome and capsid antigens in ocular samples from patients with epithelial and stromal herpetic keratitis. Methods: The polymerase chain reaction (PCR) and ELISA (Herpchek) were respectively used to detect the HSV-1 genome (DNA) and capsid antigens in corneal scrapings and tears from 8 patients with epithelial keratitis and 13 patients with inactive herpetic stromal keratitis. Results: In the epithelial keratitis samples viral DNA and antigens were detected in 6 (75%) corneal scrapings, whereas in tears viral DNA was found in 7 (88%) samples and viral antigens were not detected at all; Herpchek positive cases were also PCR positive. All stromal herpetic keratitis samples were negative both by PCR and Herpchek. Conclusions: In epithelial herpetic keratitis the presence of HSV-1 DNA should be confirmed using PCR in tear or corneal samples given its superior sensitivity to viral antigen detection using Herpchek. Since HSV-1 DNA and antigens cannot be detected in inactive stromal keratitis cases, we will explore the active entity in the future.

Keywords: herpes simplex virus • keratitis • molecular biology 
© 2003, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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