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K. Shiratori, T. Ariga, K. Ohgami, H. Kaneko, Y. Tagawa, K. Aoki, S. Ohno, Y. Shimada, S. Yamazaki, H. Ishiko; Hexon-based Phylogenetic Analysis of the Conjunctival Isolates Identified as Adenovirus Type 19 . Invest. Ophthalmol. Vis. Sci. 2003;44(13):4638.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: Adenovirus type 19 (AdV-19) and type 37 (AdV-37) are known to be causative viruses to induce epidemic keratoconjunctivitis. The discrimination of AdV serotypes between AdV-19 and AdV-37 has been difficult, because type specific antisera were not available or the quality of antisera was not suitable for test. In this study we analysed isolates identified as AdV-19 during 1985 to 2001 using phylogenetic analysis. Methods: Twenty-two isolates identified to be AdV-19 in 1985 and 1990-2001 in Sapporo, Japan were examined. All isolates were obtained from patients with epidemic keratoconjunctivitis. The 956-bp of hexon region were amplified by PCR method and determined nucleotide sequences. The sequences were compared to those of AdV-8p, 19p, 19a and 37p strains. Results: All isolates in this study were grouped into 3 groups by hexon-based phylogenetic analysis. Eleven isolates, one in 1992 and 10 in 1996-2001, formed the same cluster with AdV-19a but not AdV-19p (Group 1). Ten isolates in 1985, 90, 91, 93 and 94 formed the same cluster with AdV-37p (Group 2). One isolate in 1992 formed the same cluster with AdV-8p (Group 3). Conclusions: The above findings indicate that isolates of Group 1, 2 and 3 were identified as AdV-19a, AdV-37p and AdV-8p respectively. Strains identified as AdV-19 involved some isolates of AdV-37 at least until 1994, probably because of the quality of antisera. Hexon-based phylogenetic analysis seems useful for identification of AdV-19 and AdV-37 if type specific antisera are not available.
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