May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Molecular Eidemiology of Epidemic Keratoconjunctivitis in Austria
Author Affiliations & Notes
  • M. Akanuma
    Ophthalmology and Visual Sciences, Hokkaido University Graduate School of Medicine, Sapporo, Japan
  • T. Ariga
    Ophthalmology and Visual Sciences, Hokkaido University Graduate School of Medicine, Sapporo, Japan
  • Y. Shimada
    Research and Development, Mitsubishi Kagaku Bio-Clinical Laboratories, Tokyo, Japan
  • S. Yamazaki
    Research and Development, Mitsubishi Kagaku Bio-Clinical Laboratories, Tokyo, Japan
  • H. Ishiko
    Research and Development, Mitsubishi Kagaku Bio-Clinical Laboratories, Tokyo, Japan
  • Y. Tagawa
    Research and Development, Mitsubishi Kagaku Bio-Clinical Laboratories, Tokyo, Japan
  • K. Aoki
    Research and Development, Mitsubishi Kagaku Bio-Clinical Laboratories, Tokyo, Japan
  • S. Ohno
    Research and Development, Mitsubishi Kagaku Bio-Clinical Laboratories, Tokyo, Japan
  • M. Nagl
    Institute of Hygiene and Social Medicine, University of Innsbruck, Innsbruck, Austria
  • B. Teuchner
    University Eye Hospital Innsbruck, Innsbruck, Austria
  • Footnotes
    Commercial Relationships  M. Akanuma, None; T. Ariga, None; Y. Shimada, None; S. Yamazaki, None; H. Ishiko, None; Y. Tagawa, None; K. Aoki, None; S. Ohno, None; M. Nagl, None; B. Teuchner, None.
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 4642. doi:
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      M. Akanuma, T. Ariga, Y. Shimada, S. Yamazaki, H. Ishiko, Y. Tagawa, K. Aoki, S. Ohno, M. Nagl, B. Teuchner; Molecular Eidemiology of Epidemic Keratoconjunctivitis in Austria . Invest. Ophthalmol. Vis. Sci. 2003;44(13):4642.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To determine the serotype and genome type of epidemic keratoconjunctivitis (EKC) in Innsbruck, Austria. The study was done as a part of Worldwide Epidemiology of Infectious Conjunctivitis (WEIC). Methods: We examined a total of 60 conjunctival swabs from patients with acute conjunctivitis in Innsbruck, Austria in 2002 to detect adenovirus (AdV) by PCR method. AdV was detected in a total of 36 conjunctival swabs. Those samples were analysed to determine the serotype and genome type of AdV by PCR, PCR-RFLP and PCR sequence methods. Results: 1. The results of serotype analysis revealed that AdV-3 was found in 6 cases, AdV-4 in 3, AdV-8 in 19, AdV-19a in 4, AdV-37 in 3 and unclassified AdV in one. 2. Three isolates of AdV-3 formed the same cluster with AdV-3p whereas the rest of four formed the same cluster with AdV-3 variant found in 1999. 3. Seven isolates of AdV-8 formed the same cluster with AdV-8E, ten isolates of AdV8 formed the same cluster with AdV-8p and the rest of two isolates did not form the cluster with any genome type of AdV-8. Conclusions: The above findings indicate that AdV-8 was a predominant serotype of EKC in Innsbruck, Austria. It is known that AdV-37 was a predominant serotype of EKC in Japan in recent years. Therefore, a dominant serotype seems different between Austria and Japan.

Keywords: adenovirus • conjunctivitis • clinical (human) or epidemiologic studies: bio 
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