May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Autologous Transplantation of a Bioengineered Cultivated Oral Epithelial Cell Sheet using a Temperature-responsive Culture Surface for Ocular Surface Reconstruction
Y. Hayashida; K. Nishida; M. Yamato; K. Watanabe; N. Maeda; H. Watanabe; A. Kikuchi; T. Okano; Y. Tano
Author Affiliations & Notes
  • Y. Hayashida
    Ophthalmology, Osaka Univ Medical School, Osaka, Japan
  • K. Nishida
    Ophthalmology, Osaka Univ Medical School, Osaka, Japan
  • M. Yamato
    Institute of Biomedical Engineering, Tokyo Women’s University, Tokyo, Japan
  • K. Watanabe
    Institute of Biomedical Engineering, Tokyo Women’s University, Tokyo, Japan
  • N. Maeda
    Institute of Biomedical Engineering, Tokyo Women’s University, Tokyo, Japan
  • H. Watanabe
    Institute of Biomedical Engineering, Tokyo Women’s University, Tokyo, Japan
  • A. Kikuchi
    Institute of Biomedical Engineering, Tokyo Women’s University, Tokyo, Japan
  • T. Okano
    Institute of Biomedical Engineering, Tokyo Women’s University, Tokyo, Japan
  • Y. Tano
    Institute of Biomedical Engineering, Tokyo Women’s University, Tokyo, Japan
  • Footnotes
    Commercial Relationships  Y. Hayashida, None; K. Nishida, Cell Seed C, P; M. Yamato, Cell Seed C, P; K. Watanabe, None; N. Maeda, None; H. Watanabe, None; A. Kikuchi, None; T. Okano, Cell Seed C, P; Y. Tano, None.
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 4695. doi:
  • Views
  • Share
  • Tools
    • Alerts
      User Alerts
      You are adding an alert for:
      Autologous Transplantation of a Bioengineered Cultivated Oral Epithelial Cell Sheet using a Temperature-responsive Culture Surface for Ocular Surface Reconstruction
      You will receive an email whenever this article is corrected, updated, or cited in the literature. You can manage this and all other alerts in My Account
      The alert will be sent to:
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation
      Citation

      Y. Hayashida, K. Nishida, M. Yamato, K. Watanabe, N. Maeda, H. Watanabe, A. Kikuchi, T. Okano, Y. Tano; Autologous Transplantation of a Bioengineered Cultivated Oral Epithelial Cell Sheet using a Temperature-responsive Culture Surface for Ocular Surface Reconstruction . Invest. Ophthalmol. Vis. Sci. 2003;44(13):4695.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

      ×
    • Get Permissions
  • Supplements
Abstract

Abstract: : Purpose: The limbal epithelium is used as an autologous cell source for treating unilateral ocular surface diseases, but not bilateral diseases. In this study, we have developed a novel tissue engineering approach for treating bilateral diseases using autologous oral epithelial cells expanded on temperature-responsive cell culture surfaces. Methods: Oral mucosal epithelial cells from rabbits were cultured on a temperature-responsive culture dish with 3T3 feeder cells for 2 weeks. Cultivated cells were then harvested by reducing the temperature of the culture insert. Tissues were observed by light and electron microscopy. Autologous transplantation of cultivated oral epithelial cells was also performed in rabbits with total limbal stem cell deficiencies. Results: The rabbit oral epithelial sheet was harvested from the dish as a transplantable cell sheet simply by reducing temperature without enzymatic treatment. The bioengineered sheets were three to five cell layers thick with good evidence of tight junction and desmosome formation. By autologous transplantation, the rabbit corneal surface was successfully reconstructed and its transparency was restored. Cornea-specific keratin K3 was expressed in the bioengineered oral epithelial cell sheet when grafted. Conclusions:The corneal surface can be successfully reconstructed by autologous transplantation of our bioengineered oral epithelial cell sheet. With promising implications for bilateral ocular surface diseases.

Keywords: cornea: epithelium • transplantation • keratoprostheses 
© 2003, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2015 Association for Research in Vision and Ophthalmology.
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×
This site uses cookies. By continuing to use our website, you are agreeing to our privacy policy.Accept