Purchase this article with an account.
J. Pe'er, F.D. Raiskup, E. Binstock, A. Domb, J. Frucht-Pery; Pharmacokinetics of Gentamycin in the Rabbit Eye Administered via Iontophoresis . Invest. Ophthalmol. Vis. Sci. 2003;44(13):4761.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Purpose: Evaluation of the pharmacokinetic profile of gentamycin administered to the cornea via iontophoresis. Methods: Five groups of 8 rabbits each (one eye /rabbit) underwent general anaesthesia (inj. Ketamine and inj. Xalazine i.m.). Corneal iontophoresis using hydrogel discs containing gentamycin sulfate with current 1mAmp was applied for 1 min. Following sacrifice [immediately after the treatment (group 1), 1 hour (group 2), 2 hours (group 3), 4 hours (group 4) and 8 hours (group 5) post iontophoresis] aqueous humor was aspirated, corneas excised and gentamycin concentrations determined in the samples by TDx analysis. . Results:Concentrations of gentamycin in cornea (µg/gr): group 1(363.1±127.3); group 2 (122.1±47,1); group 3 (138.7±119.6); group 4 (47.8±19.4); group 5 (24.9± 6.1). Concentrations of gentamycin in aqueous humor (µg/ml): group 1 (3.0±3.0); group 2 (9.4± 1.5); group 3 (29.4±17.4); group 4 (13.7±10.0); group 5 (0.9± 0.2). The highest concentrations of gentamycin in cornea were found immediately after the iontophoresis and gradually declined up to the period 8 hours after the treatment. Levels of the drug 8 hours post iontophoresis were almost identical with the levels in the group (previous study) where gentamycin was administered topically by drops, every 5 min for 1 hour. The highest concentration of the drug in the aqueous humor was detected 2 hours after the iontophoresis. Conclusions:Iontophoresis using hydrogel discs immersed in gentamycin sulfate may deliver therapeutic concentrations of the drug into the cornea and anterior chamber by single administration.
This PDF is available to Subscribers Only