May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Morphological Change of Retina Caused by Subretinal Indocyanin Green in Rabbit Eyes
Author Affiliations & Notes
  • T. Kawaji
    Ophthalmology, Kumamoto Univ Sch Med, Kumamoto, Japan
  • A. Hirata
    Ophthalmology, Kumamoto Univ Sch Med, Kumamoto, Japan
  • Y. Inomata
    Ophthalmology, Kumamoto Univ Sch Med, Kumamoto, Japan
  • H. Tanihara
    Ophthalmology, Kumamoto Univ Sch Med, Kumamoto, Japan
  • Footnotes
    Commercial Relationships  T. Kawaji, None; A. Hirata, None; Y. Inomata, None; H. Tanihara, None.
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 4901. doi:
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    • Get Citation

      T. Kawaji, A. Hirata, Y. Inomata, H. Tanihara; Morphological Change of Retina Caused by Subretinal Indocyanin Green in Rabbit Eyes . Invest. Ophthalmol. Vis. Sci. 2003;44(13):4901.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: Previously we reported that persistent subretinal indocyanin green (ICG) induced retinal atrophy after macular hole surgery (Hirata,et al. Am J Ophthalmol, in press). This study was designed to investigate the effects of subretinal ICG on retinal morphology in rabbit eyes. Methods: Retinal bleb detachments, 3mm in diameter, were produced by the injection of ICG at a dose of 25 mg/ml, 5 mg/ml, 0.5 mg/ml or balanced salt solution (BSS) into the subretinal space of albino rabbit eyes using a 20G needle with 41G cannula. No vitrectomy was performed. Retinal toxicity by subretinal ICG and BSS was assessed histologically by light microscopy at 2 weeks after injection. In eyes with 5 mg/ml ICG, retinal toxicity was assessed histologically at 1 day, 3days, 1 week, 2 weeks, 1 month after injection. Results: In eyes with 25 mg/ml and 5 mg/ml ICG at 2 weeks after injection, loss of the retinal pigment epithelium and photoreceptors, and retinal atrophy was observed. In eyes with 25 mg/ml ICG, the retinal structure was destroyed more severely than with 5 mg/ml. In eyes with 5 mg/ml ICG, normal structure of the retina was preserved at 1 day after injection. However, the photoreceptors were disappeared at 3days after injection, and retinal atrophy occured. In eyes with 0.5 mg/ml ICG and BSS, normal structure of the retina was preserved and folding of the neural retina was partly observed by light microscopy. Conclusions: Subretinal ICG induces apparent morphological damage of the retina in a dose-dependent manner. ICG may be introduced into the subretinal space through an original or iatrogenic hole during vitreous surgery. Care should be taken when using intravitreous ICG.

Keywords: vitreoretinal surgery • animal model • retina 
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