May 2003
Volume 44, Issue 13
ARVO Annual Meeting Abstract  |   May 2003
Immunocytochemical Localization of Ciliary Neurotrophic Factor Receptor (CNTFR) in the Retina of Mammalian Species
Author Affiliations & Notes
  • W.A. Beltran
    James A Baker Institute for Animal Health, Cornell University, College of Veterinary Medicine, Ithaca, NY, United States
  • H. Rohrer
    Max-Planck-Institut für Hirnforschung, Frankfurt / Main, Germany
  • D. Gu
    Max-Planck-Institut für Hirnforschung, Frankfurt / Main, Germany
  • G.D. Aguirre
    Max-Planck-Institut für Hirnforschung, Frankfurt / Main, Germany
  • Footnotes
    Commercial Relationships  W.A. Beltran, None; H. Rohrer, None; D. Gu, None; G.D. Aguirre, None.
  • Footnotes
    Support  EY13132, Foundation Fighting Blindness, Morris Animal Foundation/The Seeing Eye, Inc., Van Sloun Fnd
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 4916. doi:
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      W.A. Beltran, H. Rohrer, D. Gu, G.D. Aguirre; Immunocytochemical Localization of Ciliary Neurotrophic Factor Receptor (CNTFR) in the Retina of Mammalian Species . Invest. Ophthalmol. Vis. Sci. 2003;44(13):4916.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract: : Purpose: To determine the immunolocalization of CNTFRα in the retina of several mammalian species including: mouse, rat, cat, dog, sheep, pig, horse, and human. Methods: Paraformaldehyde fixed retinas (Human: 2 % PAF for over 1 year; other species: 4 % PAF for 3 hours followed by 2 % PAF for 24 hours) were cryoprotected in gradient sucrose concentrations, embedded in OCT and sectioned. Three polyclonal antibodies directed against chick, rat or human CNTFRα were used. The anti-rat (sc-1914) and anti-human (sc-1913) CNTFRα antibodies were available commercially; the anti-chick antibody was made by one of the authors (H.R.). Results: By immunofluorescence the anti-rat and human CNTFRα antibodies labeled exclusively the photoreceptor inner (IS) and outer (OS) segments in all species but human (no label) and mouse. In mouse CNTFRα and PNA label co-localized, suggesting that expression was limited to cones. A similar pattern of staining of the IS and OS was observed using an immunoenzymatic method (ABC kit). The anti-chick CNTFRα antibody labeled (ABC method) the photoreceptor IS in all species except mouse and rat. Labeling of the cone perinuclear region, axon and synaptic terminal was observed in the dog, pig and human, and immunoreactivity of the INL, IPL, GCL and NFL was present in all species. Conclusions: Although photoreceptor IS were labeled with all three CNTFRα antibodies in most species, we observed a variation in the labeling of the inner retina. Further studies are needed to address the specificity of these antibodies in order to evaluate the expression of CNTFRα in the retina, particularly in the photoreceptor cells.

Keywords: receptors: pharmacology/physiology • retina • immunohistochemistry 

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