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E.F. van Kuijk, J.D. Moreland, A.G. Robson, D. Pauleikhoff, T. Morrissey, G.E. Holder, F.W. Fitzke, A.C. Bird; In vivo Evidence for Reduced Autofluorescence Efficiency at the Fovea: Implications for Assessment of Macular Pigment . Invest. Ophthalmol. Vis. Sci. 2003;44(13):4997.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To optimise optical density measurements of macular pigment made by fundus autofluorescence by comparison with those made by motion photometry. Methods: Fundus autofluorescence images were obtained through a high-pass barrier filter (1 x10-5 transmission at 495nm) using a modified scanning laser ophthalmoscope (488nm radiation). Optical density values were derived from fundus autofluorescence images at 12 retinal locations between 0 and 7 degrees relative to values at 7 degrees eccentricity. MP profiles derived from minimum motion matches were made in the same subjects and over the same retinal eccentricities. Results: Comparison of mean relative optical density values obtained using the psychophysical and imaging methods revealed a linear relationship over para-foveal areas (>1.8 degrees eccentricity) but deviation over central areas (<1.8 degrees). A second order polynomial regression provided the best fit for all data (r2=0.99). Differences between linear and polynomial regressions were used as an eccentricity-dependent correction. Conclusions: Assessment of MP optical density using fundus autofluorescence imaging requires the introduction of an eccentricity-dependent correction due to reduced autofluorescence efficiency at the fovea.
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