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A. Deen, S.M. Steidl; Experimentally Induced Retinal Detachment During Vitrectomy Surgery Using 2,4-Dinitrophenol . Invest. Ophthalmol. Vis. Sci. 2003;44(13):5013.
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Purpose: In some patients with severe neovascular form of age related macular degeneration, macular translocation surgery is a new surgical option. The most difficult and time-consuming step is creating the retinal detachment. We have designed a method of reproducibly creating a pharmacologically induced retinal detachment within thirty minutes of injection of 2,4-Dinitrophenol (2,4-DNP). Methods: Vitrectomies were performed in two-kg Dutch-belted rabbits. A three port pars plana core vitrectomy was performed in the both eyes of each rabbit. Using a soft tip cannula attached to a tuberculin syringe, 0.1 cc of a solution of 10mM 2,4-DNP was injected into the vitreous cavity of the right eye, through one of the sclerotomy ports. After thirty minutes, using aspiration with a soft tip cannula and a diamond dusted cannula, the loose retina at the myelin wing was gently elevated. The left eye (the control) received a vitrectomy without injection of the 2,4-DNP. Results: Within 30 minutes of injection of the 2,4-DNP in the right eye, the retina was detached, as manipulation with a cannula was sufficient to elevate the myelin wing. In the control eyes, we were unable to elevate the myelin wing with either cannula. Conclusion: We have demonstrated that an intravitreal injection of 0.1 cc of 10mM 2,4 DNP into the vitrectomized cavity of the rabbit can induce retinal detachment within thirty minutes of injection. This detachment was demonstrated by easily elevating the myelin wing using a cannula. This technique of creating a pharmacologically induced retinal detachment using 2,4-DNP might be suitable for future use in macular translocation surgery. Supported in part by an unrestricted grant from Research To Prevent Blindness.
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