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M.V. Kalayoglu, D. Bula, J. Arroyo, E.S. Gragoudas, J.W. Miller; Detection of Chlamydia pneumoniae in Choroidal Neovascular Membranes Isolated from Patients with Age-related Macular Degeneration . Invest. Ophthalmol. Vis. Sci. 2003;44(13):5023.
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Purpose: Age-related Macular Degeneration (AMD) and coronary artery disease share several risk factors, and these diseases may have similar pathogenic mechanisms that involve inflammation. The prokaryotic pathogen Chlamydia pneumoniae is an emerging risk factor in cardiovascular diseases, and recent seroepidemiological data have suggested that this pathogen also may be associated with AMD. In this study, we examined choroidal neovascular tissue (CNV) from patients with neovascular AMD for the presence of C. pneumoniae. Methods: Nine CNV removed from nine patients with neovascular AMD (ages 76-90) were formalin-fixed and paraffin-embedded. In addition, nine frozen whole eyes from patients without AMD (ages 70-85) were obtained from the New England Eye Bank; these eyes were thawed, and retina, choroid and iris were individually isolated by dissection. DNA was extracted from all tissues with the QiAamp DNA mini kit (Qiagen Inc., Valencia, CA) and amplified with touchdown-nested PCR using primers targeting the C. pneumoniae ompA gene (CP1-CP2/CPC-CPD). Samples were electrophoresed on 1% agarose gels, stained with ethidium bromide and visualized under ultraviolet light. Results: C. pneumoniae DNA was amplified from two of nine CNV and none of the non-AMD eyes. Positive samples were subjected to automated sequencing and confirmed to have identity to the targeted C. pneumoniae ompA gene. Conclusions: These data indicate that C. pneumoniae DNA is present in some CNV secondary to AMD, and suggest that C. pneumoniae infection may be associated with the development of AMD.
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