May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Suprachoroidal-Transretinal Stimulation (STS) Can Elicit Localized Evoked Responses From the Superior Colliculus in Normal and RCS Rats
H. Kanda; H. Sawai; T. Morimoto; T. Fujikado; Y. Tano; Y. Fukuda
Author Affiliations & Notes
  • H. Kanda
    Physiology and Biosignaling, Osaka Univ. Grad. Sch. Med., Suita, Japan
  • H. Sawai
    Physiology and Biosignaling, Osaka Univ. Grad. Sch. Med., Suita, Japan
  • T. Morimoto
    Physiology and Biosignaling, Osaka Univ. Grad. Sch. Med., Suita, Japan
  • T. Fujikado
    Visual Science, Osaka Univ. Grad. Sch. Med., Suita, Japan
  • Y. Tano
    Ophthalmology, Osaka Univ. Grad. Sch. Med., Suita, Japan
  • Y. Fukuda
    Ophthalmology, Osaka Univ. Grad. Sch. Med., Suita, Japan
  • Footnotes
    Commercial Relationships  H. Kanda, None; H. Sawai, None; T. Morimoto, None; T. Fujikado, None; Y. Tano, None; Y. Fukuda, None.
  • Footnotes
    Support  Research on Eye and Ear Sciences H13-014
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 5053. doi:
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      H. Kanda, H. Sawai, T. Morimoto, T. Fujikado, Y. Tano, Y. Fukuda; Suprachoroidal-Transretinal Stimulation (STS) Can Elicit Localized Evoked Responses From the Superior Colliculus in Normal and RCS Rats . Invest. Ophthalmol. Vis. Sci. 2003;44(13):5053.

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Abstract

Abstract: : Purpose: In order to minimize insult to the retina while implanting stimulating electrodes, we developed a new stimulation method for the retinal prosthesis, named suprachoroidal-transretinal stimulation (STS). To evaluate whether the STS is appropriate to evoke artificial vision, we measured threshold intensity of the evoked potentials (EPs) from the superior colliculus (SC) and their localization in normal and Royal College of Surgeons (RCS) rats. Methods: Female hooded rats (Long Evans, n=17) with ages 8-14 weeks and RCS rats (n=6) with ages 25-30 weeks were used. The animals were anesthetized and maintained with urethane (1.75g/kg, i.p.). Every procedure conformed to the Guideline of ARVO. A small fenestration (diameter: about 0.5mm) was made in upper temporal part of the sclera. For electrical stimulation, a silver ball electrode (diameter: 0.2-0.3 mm) was placed on the fenestration. Another electrode of epoxy coating stainless wire (diameter: 0.5mm), with an exposed tip of about 0.2mm, was inserted into the vitreous. Between the two electrodes electrical pulses of 5-100 µA in amplitude and 0.05-0.5ms in duration were applied. The EPs were recorded from the SC with another silver ball electrode (diameter: 0.2 mm) which was systematically moved with a micromanipulator to cover an area of 0.5×0.5mm – 1.0×1.0mm on the SC surface. Results: In response to STSs triphasic EPs appeared having similar wave forms to those evoked by stimulation of the optic nerve. The threshold intensity of the STS was about 5-10nC in both normal and RCS rats. The EPs to suprathreshold STSs were localized in a collicular surface area of 0.12+/-0.07mm2. In all cases the EP with a maximum amplitude was obtained from the SC area that represents the locus of retina that was stimulated with the STS. Conclusions: The STS can focally activate retinal ganglion cells with reasonable currents in normal rats as well as in RCS rats. The STS is less invasive to the retina than other methods of retinal stimulation such as epiretinal and subretinal implants. Therefore, we conclude that the STS method is suitable for designing a new type of retinal prosthesis.

Keywords: electrophysiology: non-clinical • retina: proximal(bipolar, amacrine, and gangli • superior colliculus/optic tectum 
© 2003, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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