Abstract
Abstract: :
Purpose: To study photo toxicity by camera flashlight for eye. Methods: Right eyes of Sprague-Dawley rats were exposed to electronic flash in different distance (0.1 m, 1m or 3m) and counts (10, 100, 1000) with stimulus interval of 5 sec (n=2 for each group). Single-flash ERG was recorded from both eyes before and 24 hours after light exposure under dark-adapted condition, compared to other control eyes. Corneal epithelial damage was assessed with fluorescein staining which was scored from 0 to 9, given as a sum of the score grading 0 to 3 in each area of upper, middle and lower cornea. After eyes were enucleated, cornea was processed for scanning electron microscopy (SEM) and posterior parts of eyes were processed for paraffin embedding for light microscopy. Results: In the maximum exposure condition (i.e. in 0.1m with 1000 counts), the amplitude of ERG was reduced to 77% in a-wave and 80% in b-wave in comparison with the fellow eye, while no significant change (96 – 104% for a-wave and 94– 107% for b-wave) was found in other conditions. No difference in amplitude of ERG was detected in each eye before light exposure. The fluorescein score ranged 0 to 2 except in the maximum condition where the score was 6 and detached epithelial cells were detected by SEM. No morphological changes in retina were seen in any eyes with each light exposure condition. Conclusions: Photo toxicity of camera flashlight is minimal to retina and cornea in regular use.
Keywords: stress response • electroretinography: non-clinical • retina