May 2003
Volume 44, Issue 13
ARVO Annual Meeting Abstract  |   May 2003
Inhibitory Effect of Cannabinoids on [3H]D-aspartate Release from Bovine Isolated Retinae
Author Affiliations & Notes
  • C.A. Opere
    Pharmacy Sciences, Creighton University, Omaha, NE, United States
  • K.H. Kulkarni
    Pharmacy Sciences, Creighton University, Omaha, NE, United States
  • S.E. Ohia
    School of Pharmacy, University of huston, Huston, TX, United States
  • Footnotes
    Commercial Relationships  C.A. Opere, None; K.H. Kulkarni, None; S.E. Ohia, None.
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 5139. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      C.A. Opere, K.H. Kulkarni, S.E. Ohia; Inhibitory Effect of Cannabinoids on [3H]D-aspartate Release from Bovine Isolated Retinae . Invest. Ophthalmol. Vis. Sci. 2003;44(13):5139.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Abstract: : Purpose: Although cannabinoid receptors are present in the mammalian retina (Lu et al., Vis. Neurosc. 17: 91, 2000), their role in neurocircuitry and neurotransmission remains unknown. In the present study, we evaluated the effect of cannabinoid CB-receptor agonists and antagonists on potassium (K+)-induced [3H]D-aspartate release from bovine retinae in vitro. Methods: Isolated neural retinae were incubated in oxygenated Krebs solution containing 200 nM of [3H]D-aspartate for 60 mins and then prepared for studies of neurotransmitter release using the superfusion method. Release of [3H]D-aspartate was evoked by K+(50 mM) stimuli applied at 90 mins (S1) and at 108 mins (S2) after the onset of superfusion. Under these experimental conditions, [3H]D-aspartate has been reported to be a valid marker of endogenous glutamate in retinal neurons (Santos et al., Neurochem. Res. 21: 361, 1996). Results: In the concentration range, 0.1 to 10 µM, the cannabinoid CB-receptor agonists methanandamide, arachidonyl-2'-chloroethylamide (ACEA) and WIN 55,212-2 caused a concentration-dependent inhibition of K+-induced [3H]D-aspartate overflow without affecting basal tritium efflux. For instance, at 10 µM, ACEA and methanadamide attenuated [3H]D-aspartate release by 37% and 20%, respectively. The cannabinoid CB1-receptor selective antagonist, N-(Piperidin-1-yl)-5-(4-iodophenyl)-1-(2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide (AM-251) completely reversed the effects of methanadamide (1 µM) and ACEA (1 µM) on K+-induced [3H]D-aspartate release. Conclusions: We conclude that CB1-receptors mediate the inhibitory action of cannabinoids on K+-induced [3H]D-aspartate release from bovine isolated retinae.

Keywords: neurotransmitters/neurotransmitter systems • pharmacology • retina: neurochemistry 

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.