May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Neuroprotective Effect of Lens Epithelium-Derived Growth Factor (LEDGF) on N-Methyl-D-Aspartate-Induced Cell Death in Rat Retina
Y. Inomata; A. Hirata; T. Koga; T. Shinohara; D.P. Shingh; A. Kimura; H. Tanihara
Author Affiliations & Notes
  • Y. Inomata
    Ophtalmology, Kumamoto univ. School of Med., Kumamoto, Japan
  • A. Hirata
    Ophtalmology, Kumamoto univ. School of Med., Kumamoto, Japan
  • T. Koga
    Ophtalmology, Kumamoto univ. School of Med., Kumamoto, Japan
  • T. Shinohara
    Ophtalmology, University of Nebraska Medical Center, Omaha, NE, United States
  • D.P. Shingh
    Ophtalmology, University of Nebraska Medical Center, Omaha, NE, United States
  • A. Kimura
    Ophtalmology, University of Nebraska Medical Center, Omaha, NE, United States
  • H. Tanihara
    Ophtalmology, University of Nebraska Medical Center, Omaha, NE, United States
  • Footnotes
    Commercial Relationships  Y. Inomata, None; A. Hirata, None; T. Koga, None; T. Shinohara, None; D.P. Shingh, None; A. Kimura, None; H. Tanihara, None.
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 5206. doi:
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      Y. Inomata, A. Hirata, T. Koga, T. Shinohara, D.P. Shingh, A. Kimura, H. Tanihara; Neuroprotective Effect of Lens Epithelium-Derived Growth Factor (LEDGF) on N-Methyl-D-Aspartate-Induced Cell Death in Rat Retina . Invest. Ophthalmol. Vis. Sci. 2003;44(13):5206.

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Abstract

Abstract: : Purpose: To determine if lens epithelium-derived growth factor (LEDGF) has a neuroprotective effect against N-methyl-D-aspartate (NMDA)-induced cell death in retina. Methods: 6-week-old Sprague-Dawley (SD) rats received an intravitreal injection of LEDGF fused with glutathione-S-transferase (GST-LEDGF). Fellow eyes received vehicle as control. 7, 4 and 2 days after injection, same time, and 1day before injection of GST-LEDGF, eyes of rats were administered with NMDA (total amount of 20nmol) into the vitreous cavity. Each eye was enucleated, then transverse sections were subjected to morphometric analysis. Retrograde labeling with a fluorescent tracer (fluoro-Gold) was applied for counting survived retinal ganglion cells (RGCs) 4days after NMDA injection. Retinal damage was assessed by TUNEL-method 18 hours after NMDA injection. Western blotting analysis was perfomed for examination of heat shock protein 25 (Hsp25) and aB-crystallin in GST-LEDGF injected retina. Results: Pretreatment with GST-LEDGF 4 and 2 days before administration of NMDA provided a significant neuroprotective effect against NMDA-induced retinal damage. Retrograde labeling experiments revealed pretreatment with GST-LEDGF at 2days before NMDA injection induced neuroprotective effect of RGCs. In TUNEL method, it revealed that pretreatment with GST-LEDGF prevents NMDA induced apoptosis. Western blotting studies demonstrated upregulation of Hsp25 andaB-crystallin expression in retina of LEDGF injection from 24 to 48 hours. Conclusions: Present studies suggest that GST-LEDGF provides neuroprotective effects on RGCs against NMDA-induced retinal damage. The increased expression of Hsp25 and aB-crystallin may play a crucial role of this effects.

Keywords: neuroprotection • growth factors/growth factor receptors • retina: proximal(bipolar, amacrine, and gangli 
© 2003, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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