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M.H. Sun, K.J. Chen, W.C. Wu, C.C. Lai, L.Y. Kao, Y.P. Tsao; Adeno-associated Virus Vector Expressing Glial Cell Lined-derived Neurotrophic Factor Enhances Retinal Ganglion Cell Survival in Axotomized Rats . Invest. Ophthalmol. Vis. Sci. 2003;44(13):5225.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: to determine whether glial cell lined-derived neurotrophic factor ( GDNF ) enhances retinal ganglion cell survival in rats with optic nerve transection injury model. Methods: transfection of rAAV-GDNF to ganglion cells was achived by intravitreal injection in adult albino Lewis rats for 3 weeks. The optic nerves were transected intraorbitally about 2 mm from the eye . Two weeks later the neuronal loss was determined by retinal ganglion cell counts ( RGCCs ) through FITC-Dexan back-labeling. Results: RGCCs in AAV-GDNF-treated retina was 380.4cells/mm2 , RGCCs in AAV-lacZ -treated retina was 205.2 cells/mm2 . Conclusion: The preliminary result showed AAV-GDNF exerts an RGC rescue rate of about 40% at 14-days post-axotomy
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