May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Adeno-associated Virus Vector Expressing Glial Cell Lined-derived Neurotrophic Factor Enhances Retinal Ganglion Cell Survival in Axotomized Rats
M.H. Sun; K.J. Chen; W.C. Wu; C.C. Lai; L.Y. Kao; Y.P. Tsao
Author Affiliations & Notes
  • M.H. Sun
    Ophthalmology, Chung Gung Memorial Hosp, Kwei-Shan, Taiwan Republic of China
  • K.J. Chen
    Ophthalmology, Chung Gung Memorial Hosp, Kwei-Shan, Taiwan Republic of China
  • W.C. Wu
    Ophthalmology, Chung Gung Memorial Hosp, Kwei-Shan, Taiwan Republic of China
  • C.C. Lai
    Ophthalmology, Chung Gung Memorial Hosp, Kwei-Shan, Taiwan Republic of China
  • L.Y. Kao
    Ophthalmology, Chung Gung Memorial Hosp, Kwei-Shan, Taiwan Republic of China
  • Y.P. Tsao
    Ophthalmology, Chung Gung Memorial Hosp, Kwei-Shan, Taiwan Republic of China
  • Footnotes
    Commercial Relationships  M.H. Sun, None; K.J. Chen, None; W.C. Wu, None; C.C. Lai, None; L.Y. Kao, None; Y.P. Tsao, None.
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 5225. doi:
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      M.H. Sun, K.J. Chen, W.C. Wu, C.C. Lai, L.Y. Kao, Y.P. Tsao; Adeno-associated Virus Vector Expressing Glial Cell Lined-derived Neurotrophic Factor Enhances Retinal Ganglion Cell Survival in Axotomized Rats . Invest. Ophthalmol. Vis. Sci. 2003;44(13):5225.

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Abstract

Abstract: : Purpose: to determine whether glial cell lined-derived neurotrophic factor ( GDNF ) enhances retinal ganglion cell survival in rats with optic nerve transection injury model. Methods: transfection of rAAV-GDNF to ganglion cells was achived by intravitreal injection in adult albino Lewis rats for 3 weeks. The optic nerves were transected intraorbitally about 2 mm from the eye . Two weeks later the neuronal loss was determined by retinal ganglion cell counts ( RGCCs ) through FITC-Dexan back-labeling. Results: RGCCs in AAV-GDNF-treated retina was 380.4cells/mm2 , RGCCs in AAV-lacZ -treated retina was 205.2 cells/mm2 . Conclusion: The preliminary result showed AAV-GDNF exerts an RGC rescue rate of about 40% at 14-days post-axotomy

Keywords: neuroprotection • gene transfer/gene therapy • ganglion cells 
© 2003, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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