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T. Seki; Effects of Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) on the survival of Ganglion Cell Death in the Rat Retina after Cutting Optic Nerve . Invest. Ophthalmol. Vis. Sci. 2003;44(13):5226.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: We have reported already that PACAP (Pituitary Adenylate Cyclase-Activating Polypeptide) prevents from rerinal ganglion cell death using ischemia-repefusion model by a rat intra ocular hypertension. We examined effects of prevention from delayed neuronal cell death by PACAP using an optic nerve cutting model of the rat. Methods: We made a dissection of optic nerve cutting model, and the 3 micro L of the control medium(vehicle) or PACAP was administered in a vitreous body. At 7th day after the treatment, eyes were enucleated and posterior segments were fixed immediately. The retinal ganglion cell numbers were counted on the light microsope. The data was analyzed by student-t (two-tailed) between vehicle group or PACAP group and the no treatment group. And the data between PACAP groups and vehicle group were examined using Dunnet (two-tailed). Results: The number of survival ganglion cells decreased apparently in the injected vehicle group compared with the no cutting treatment. In the group which injected 10pmol/3microL and 100pmol/3microL with PACAP, the numbers of survival ganglion cells were statistically larger than the group of the vehicle injected into the vitreous body in this model. Conclusions: These findings suggest that PACAP prevents apoptosis of ganglion cells from insult in cutting optic nerve.
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