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R. Inoue, H. Yamada, Y. Suzuki, M. Araie, K. Kashiwagi; Neurotrophic Factors’ mRNAs and Proteins Are Up Regulated by Betaxolol and Calcium Channel Blocker in Cultured Muller Cell . Invest. Ophthalmol. Vis. Sci. 2003;44(13):5240.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: Muller cell is reported to produce neurotrophic factors and may be a part of neuroprotective system in the retina. The aim of this study was to investigate the change of neurotrophic factor mRNA and protein expression of cultured Muller cell by adding anti-glaucoma drugs and calcium channel blocker to culture medium. Methods: Muller cells from 3-day-old Wister rat were isolated and cultured with DMEM with 10% fetal bovine serum. After four time passages, timolol (beta-blocker, 0.1microM, 0.01microM, 0.001microM), betaxolol (beta-blocker, 0.1microM, 0.01microM, 0.001microM), bunazosin (alpha1-blocker, 0.1microM, 0.01microM, 0.001microM), and iganidipine (calcium channel blocker, 0.01microM, 0.001microM) were added to culture medium. mRNA and protein were extracted 6 hours after the application of drugs and the expression levels of neurotrophic factors were evaluated. The expression level of mRNA and protein of brain-derived neurotrophic factor (BDNF), ciliary neurotrophic factor (CNTF), and glial cell line-derived neurotrophic factor (GDNF) was examined using the reverse transcriptase-polymerase chain reaction (RT-PCR), real-time PCR and Western blot analysis. Results: Real-time PCR showed betaxolol (0.1microM, 0.01microM) up-regulated BDNF and CNTF mRNA and iganidipine (0.01microM) up-regulated BDNF, CNTF and GDNF mRNA. Western blot analysis showed betaxolol (0.1microM) and iganidipine (0.01microM) up-regulated BDNF and CNTF protein. Timolol and bunazosin did not up-regulate neurotrophic factors. Conclusions: Betaxolol and calcium channel blocker, iganidipine up-regulated neurotrophic factors in cultured Muller cells. These observations suggest that calcium channel blocker may protect retinal ganglion cell by up-regulating neurotrophic factors’ expression in Muller cell.
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