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X.D. Sun, X. Zhang, X. Xu, H.F. Lu, R.J. Chen; Effect on Apoptosis by Nerve Growth Factor in Experiment Retinal Detachment . Invest. Ophthalmol. Vis. Sci. 2003;44(13):5244.
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Purpose:To study the protective effect and mechanism by nerve growth factor (NGF) on apoptosis in experimental retinal detachment (RD). Methods:Eighty two Sprague-Dawely rats were used as RD animal model by infused 0.1% sodium hyaluronate under the neurosensory retina. Those rats were divided into NGF group and control group. NGF 5µg/5µl/eye and PBS 5µl/eye as control were respectively injected into vitreous every 4 days after RD. On postoperative time (hours and days) 1.5h, 3h, 6h, 12h, 1d, 2d, 4d, 8d, 16d, 32d, SD rats were sacrificed and eyes were extracted. The effect of NGF on retina was assessed by electron, light microscopy and cell counter. Apoptosis was investigated by TUNEL technique and electron microscopy, mRNA expression of c-fos gene was detected by RT-PCR apoptosis related gene were identified by immunohistochemistry. SPSS 10.0 was used as statistical analysis. Results:Apoptosis was observed in the two groups, which showed lower incidence rate of apoptosis in NGF groups than in control groups through apoptosis cell counter studies (p<0.05). mRNA expression of c-fos gene was detected in early RD days. Further studies revealed that the expressions of Fas-L, c-fos, caspase-3 were attenuated and bcl-2 was upregulated in NGF groups (p<0.05). Conclusions:Intravitreal injection exogenous NGF partly protect retinal cells from apoptosis in experimental RD. The neuroprotective effects of NGF are partly ascribed to its attenuation of ligand of death receptor Fas/Apo-1 expression, intervention of c-fos gene and bcl-2 gene expression, which inhibit the activation of caspase-3 and interrupt the course of apoptosis as the result.
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