December 2002
Volume 43, Issue 13
ARVO Annual Meeting Abstract  |   December 2002
Assessment of TFF Gene Expression in Normal and Dry Eye Conjunctiva
Author Affiliations & Notes
  • PO Lafontaine
    Ophthalmology Chu-Dijon Dijon France
  • N Buron
    INSERM U 517 Dijon France
  • E Solary
    INSERM U 517 Dijon France
  • AM Bron
    Ophthalmology Chu-Dijon Dijon France
  • C Garcher
    Ophthalmology Chu-Dijon Dijon France
  • Footnotes
    Commercial Relationships   P.O. Lafontaine, None; N. Buron, None; E. Solary, None; A.M. Bron, None; C. Garcher, None.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 101. doi:
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    • Get Citation

      PO Lafontaine, N Buron, E Solary, AM Bron, C Garcher; Assessment of TFF Gene Expression in Normal and Dry Eye Conjunctiva . Invest. Ophthalmol. Vis. Sci. 2002;43(13):101.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract: : Purpose: Trefoil Factors (TFF) are the secretory products of mucous epithelia. They are co-expressed with and act in concert with mucins. Our aim was to compare TFF1 (pS2) expression in normal human conjunctiva and in dry eye (DE). Methods: Nine impression cytology specimens were collected in patients with severe dry eye with Sjögren's syndrome(SS). Ten normal subjects were enrolled to provide normal control values. We assessed the expression of TFF1 (pS2) mRNA by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). Results: We found a statistically significantly lower expression of TFF1 in patient with DE than in normal conjunctiva (p<0,0001 ; Mann and Whitney U=30). TFF1 mRNA expression was close to zero in patient with DE, except for two specimens. Conclusion: A significant decrease of TFF1 mRNA expressions by epithelial cells was consistently found in the DE groups compared with in normal eyes. This result should be related to the decreased number of goblet cells.

Keywords: 365 conjunctiva 

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