December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Tear IgE Level Measurement Using Semiquantitative Methods in Children With Vernal Keratoconjunctivitis (VKC)
Author Affiliations & Notes
  • S Doan
    Ophthalmology Hosp Bichat Paris France
  • N Belayachi
    Ophthalmology Hosp Bichat Paris France
  • L Ren
    Ophthalmology Hosp Bichat Paris France
  • T Hoang-Xuan
    Hosp Bichat Ophthalmology Paris France
  • Footnotes
    Commercial Relationships   S. Doan, None; N. Belayachi, None; L. Ren, None; T. Hoang-Xuan, None.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 114. doi:
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      S Doan, N Belayachi, L Ren, T Hoang-Xuan; Tear IgE Level Measurement Using Semiquantitative Methods in Children With Vernal Keratoconjunctivitis (VKC) . Invest. Ophthalmol. Vis. Sci. 2002;43(13):114.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To compare 2 semiquantitative methods of total tear IgE level measurement in VKC patients. Methods: Twenty patients (28 eyes) with VKC were included.Two commercially available kits were tested : Stallerdiag (Stallergènes, France) and Lacrytest (Adiatec, France). Tears were collected with a paper strip in the inferior conjunctival fornix, in a standardized manner. Total IgE levels were measured with Stallerdiag and Lacrytest by an ELISA technique and a colloid gold labeling technique, respectively. Lacrytest allowed an immediate assessement of the result. Threshold detection level was 2.5 kIU/L with Lacrytest and 1.7 kIU/L with Stallerdiag. Results: Results were consistent in 22/28 cases (79%) and different in 6/28 cases (21%). Both test were significantly correlated (K = 0.55). IgEs were detected in 64% and 57% of patients with Lacrytest and Stallerdiag tests, respectively. Conclusion: Stallerdiag and Lacrytest tests are comparable and easy to use for total tear IgE level assessment.

Keywords: 437 inflammation • 366 conjunctivitis 
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