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Y Sano, N Fullwood, Y Ishino, S Hyon, D-H Kim, H Tokushige, S Kimura, Y Kawamoto, S Kinoshita; Morphological Analysis of Polyphenol Treated Rat Corneal Endothelium in Long-Term Storage . Invest. Ophthalmol. Vis. Sci. 2002;43(13):139.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To examine the effects of polyphenol treatment in Optisol-GS on long-term corneal storage at 4C. Methods:Corneal tissues obtained from Wistar rats were treated with polyphenol (500 µg/ml) at 4C for 24 hours. After treatment, the corneal tissues were stored at 4C for 28 days in Optisol-GS medium. The surface of corneal endothelial cells were examined histologically by scanning electron microscopy (SEM) and analyzed for endothelial cell density (ECD), coefficient of variation of cell size (CV), and percentage of hexagonal cells (Hex). The ultrastructure of the endothelial cells and cellular organelles was also examined by transmission electron microscopy (TEM). Results: The ECD, CV, and Hex of polyphenol treated corneas were almost equivalent to those of freshly obtained normal cornea, even after 28 days preservation in Optisol-GS. However, untreated corneas preserved in Optisol-GS for 28 days could not be analyzed due to their poor morphological appearance, as revealed by SEM. TEM showed the ultrastructure of the endothelial cells with polyphenol treatment was better than those preserved in Optisol-GS alone. In the polyphenol treated cells the mitochondria and the nuclear membranes were well preserved. In the Optisol-GS only treated samples, the mitochondria and the nuclear membranes were clearly disrupted. Conclusion:These results indicate that polyphenol treatment of corneal tissue may be beneficial for the maintenance of endothelial cell morphology during long-term preservation.
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