December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
In Herpetic Cornea, Inflammatory Cytokines Predominate, While Th1 Cytokines Hold Virus Spread in the Trigeminal Ganglion
Author Affiliations & Notes
  • C Sotozono
    Ophthalmology Kyoto Prefectural Univ of Medicine Kyoto Japan
  • S Kinoshita
    Ophthalmology Kyoto Prefectural Univ of Medicine Kyoto Japan
  • T Inoue
    Ophthalmology Osaka Univ Medical School Osaka Japan
  • Y Inoue
    Ophthalmology Tottori Univ Medical School Tottori Japan
  • Y Shimomura
    Ophthalmology Kinki Univ School of Medicine Osaka Japan
  • K Hayashi
    Kobe Institute of health Hyogo Japan
  • Footnotes
    Commercial Relationships   C. Sotozono, None; S. Kinoshita, None; T. Inoue, None; Y. Inoue, None; Y. Shimomura, None; K. Hayashi, None. Grant Identification: support:Japanese Ministry of Education (11771066)
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 39. doi:
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    • Get Citation

      C Sotozono, S Kinoshita, T Inoue, Y Inoue, Y Shimomura, K Hayashi; In Herpetic Cornea, Inflammatory Cytokines Predominate, While Th1 Cytokines Hold Virus Spread in the Trigeminal Ganglion . Invest. Ophthalmol. Vis. Sci. 2002;43(13):39.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To compare cytokine expression levels between cornea and trigeminal ganglion in herpetic keratitis, and to examine the relation between viral replication and cytokine expression. Methods: . Balb/c mouse (8 weeks old, female) corneas were scarified and infected with HSV-1 (CHR3 strain 1X106 pfu/ml, N=108). At 4, 8 and 12 days post-infection (PI), 10 types of cytokines (IL-1alpha, -1beta, -2, -4, -6, -10, -12, -18, IFN-gamma, and TNF-alpha) in the cornea and trigeminal ganglion were quantitated by ELISA. The results were superimposed on the clinical scores and virus titers for corneas and trigeminal ganglia. Results: In the cornea, proinflammatory cytokines (IL-1alpha, IL-1beta and IL-6) predominated, reaching maximum at 12 days PI, while in the trigeminal ganglion, Th1 type cytokines (IL-2, IFN-gamma and IL-12) were preeminent at 4-8 days PI and 12 days PI respectively. Th2 type cytokines (IL-4 and IL-10) were below the detection limit of ELISA. Virus titer was maximum at 4 days PI in the cornea and at 5 days PI in the trigeminal ganglion. Stromal opacities appeared at 2 days PI and persisted. Conclusion: In the herpetic cornea, inflammatory cytokines were predominent and were responsible for cell infiltrations and corneal opacities. By contrast, Th1 type cytokines played dominant roles in the trigeminal ganglion, and were considered responsible for the maintenance of virus latency. Supported by research grant 11771066 from the Japanese Ministry of Education. None.

Keywords: 370 cornea: basic science • 380 cytokines/chemokines • 425 herpes simplex virus 
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