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AM Rosen, DB Denham, F Manns, V Fernandez, C Billotte, J-M Parel; Method For Assessing Changes In Lens Volume During Immersion In Tissue Preservation Media . Invest. Ophthalmol. Vis. Sci. 2002;43(13):448.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To find an isotonic solution to preserve the human crystalline lens. Methods: BSS, Ringer's, Optisol, and Dulbecco's Modified Eagle Medium solutions differing in osmolality were selected. Human cadaver lenses were extracted by either cutting or dissolving the zonules with a-chymotrypsine. A custom-made PMMA cell was constructed to hold the lens. The cell was placed in a modified 20X optical comparator, and the lens diameter and thickness profiles were measured by hand with a ruler on the comparator screen and photographed with a digital camera at 15-minute intervals over a period of 5 hours. The digital images were then analyzed using a computer program. The volume of the lens at each interval was then calculated, assuming rotational symmetry, by using the equation for the volume of an ellipsoid. The changes in diameter, thickness, and volume versus time were analyzed to compare the effect of each solution. Results from the manual measurements were compared to those from the computer analysis to determine which technique was more accurate. Results: The measurement resolution for the manual and digital techniques were 25mm and 13mm, respectively, with volumetric resolutions of 1.0% and 0.5%. A 42 year old lens (OD) with an initial volume of 210ml increased by 8 ml (3.7%) over 5 hrs in BSS whereas, the contra lateral lens (OS) with an initial volume of 221ml increased by 3ml (1.5%) over the same time period in Ringer's. Conclusion: The optical comparator system can precisely assess the effect of preservation mediums on the physical dimensions of immersed lenses. Support: Henri and Flore Lesieur Foundation; Florida Lions Eye Bank; Research to Prevent Blindness; Australian Cooperative Research Centre (CRC) Scheme, CRC for Eye Research and Technology, Sydney.
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